Tanabe Soichi, Hase Makiko, Yano Takeo, Sato Masahiko, Fujimura Tatsuya, Akiyama Hiroshi
Graduate School of Biosphere Science, Hiroshima University, Hiroshima, Japan.
Biosci Biotechnol Biochem. 2007 Dec;71(12):3131-5. doi: 10.1271/bbb.70683. Epub 2007 Dec 7.
A rapid real-time quantitative PCR method to detect trace amounts of pork, chicken, beef, mutton, and horseflesh in foods was developed. The primers and TaqMan MGB (minor groove binder) probes were designed on the gene encoding cytochrome b for the specific detection of each species. The limit of quantification of this method was found to be 100 fg/microl of each mitochondrial DNA in 10 ng/microl of the wheat mitochondrial DNA matrix. The calculated R(2) values of the standard curves for the five species ranged between 0.994 and 0.999. This method would be particularly useful in the detection of hidden meat mince in processed foods, which would verify food labeling and gain consumers' trust.
开发了一种快速实时定量PCR方法，用于检测食品中痕量的猪肉、鸡肉、牛肉、羊肉和马肉。针对编码细胞色素b的基因设计了引物和TaqMan MGB（小沟结合物）探针，以特异性检测每个物种。该方法的定量限为在10 ng/μl小麦线粒体DNA基质中每种线粒体DNA为100 fg/μl。五个物种标准曲线的计算R(2)值在0.994至0.999之间。该方法在检测加工食品中隐藏的肉馅方面将特别有用，这将验证食品标签并赢得消费者的信任。
