Moya S L, Giuliani M G, Santini M S, Quintana M G, Salomón O D, Liotta D J
Instituto Nacional de Medicina Tropical (INMeT-MSAL), Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
Instituto Nacional de Medicina Tropical (INMeT-MSAL), Argentina.
Acta Trop. 2017 Aug;172:122-124. doi: 10.1016/j.actatropica.2017.05.004. Epub 2017 May 2.
In Puerto Iguazú City, Argentina, human and canine Visceral Leishmaniasis cases have been recorded since the year 2010, with Leishmania infantum as the etiological agent and Lutzomyia longipalpis as its main vector. In the present study, polymerase chain reaction and sequencing were used to detect L. infantum DNA in 3.9% of the female sandflies captured in Puerto Iguazú City. This is the first report of L. infantum DNA detection in Micropygomyia quinquefer, and the second one in Lu. longipalpis and Nyssomyia whitmani for Argentina. Although the detection of Leishmania DNA itself is not enough to determine a Phlebotomine species as a vector, these results are significant in setting the direction of further investigations of vectorial competence and capacity, necessary to define the roles of different sandflies species as specific or permissive vectors in the transmission VL cycle.
在阿根廷的伊瓜苏港市，自2010年以来已记录到人类和犬类内脏利什曼病病例，病原体为婴儿利什曼原虫，主要传播媒介为长须罗蛉。在本研究中，采用聚合酶链反应和测序方法，在伊瓜苏港市捕获的雌性白蛉中，有3.9%检测到婴儿利什曼原虫DNA。这是在五鬃微小白蛉中首次检测到婴儿利什曼原虫DNA的报告，也是阿根廷在长须罗蛉和惠氏罗蛉中第二次检测到该DNA。尽管仅检测到利什曼原虫DNA不足以确定白蛉物种为传播媒介，但这些结果对于确定进一步研究媒介能力和容量的方向具有重要意义，而这对于确定不同白蛉物种在内脏利什曼病传播循环中作为特定或允许传播媒介的作用是必要的。
