Thomaz-Soccol Vanete, Gonçalves André Luiz, Baggio Rafael Antunes, Bisetto Alceu, Celestino Adão, Hospinal-Santiani Manuel, de Souza André, Mychalizen Mario, Borges Marcelo Eduardo, Piechnik Cláudio Adriano
Federal University of Paraná (UFPR), Molecular Biology Laboratory, Department of Bioprocess Engineering and Biotechnology, Rua Francisco H. dos Santos, Centro Politécnico, Curitiba, PR, Brazil.
SESA- Secretary of Health of the State of Paraná and the Ninth Health Region, Paraná, Brazil.
One Health. 2023 Jun 9;17:100581. doi: 10.1016/j.onehlt.2023.100581. eCollection 2023 Dec.
The recent geographic spread of along the borders of Argentina, Brazil and Paraguay has been highlighted. In our previous study, was found in 55 of 123 patches surveyed, and in some patches, sandflies were found at higher densities, forming hotspots. Based on the One Health approach, we investigated the seasonality of the vector, the presence of parasite DNA, and the environmental factors that contribute to vector and parasite dispersal in these previously described hotspots in Foz do Iguaçu, Brazil. Entomological surveys were conducted monthly for one year. Fourteen hotspots peridomicile and six intradomicile were sampled. PCR was used to assess the prevalence of DNA in sandflies. Zero-inflated negative binomial regression was used to determine the association of micro- and mesoscale environmental variables with the occurrence and abundance of the three most abundant sandfly species sampled. A total of 3543 species were captured, with being the predominant species (71.78%) of the 13 species found. , , and were reported for the first time in the region. NDVI, distance to water, precipitation, west-to-east wind, wind speed, maximum and minimum relative humidity, and sex were significant variables associated with vector presence/abundance in the environment. Vector presence/abundance in the peridomicile was associated with precipitation, altitude, maximum temperature, minimum and maximum relative humidity, west-to-east wind, wind speed, and sex. DNA was detected in an average of 21% of throughout the year. Vector abundance is concentrated in urban and peri-urban areas, with some specimens present in different parts of the city and some sites with high vector abundance. This distribution suggests that the risk of actual contact between humans and parasite vectors in urban areas during the epidemic period is associated with patches of peri-urban vegetation and then extends into urban areas.
近期 在阿根廷、巴西和巴拉圭边境地区的地理扩散情况已受到关注。在我们之前的研究中，在所调查的123个区域中有55个发现了 ，并且在一些区域中，白蛉的密度更高，形成了热点地区。基于“同一健康”方法，我们在巴西伊瓜苏港这些先前描述的热点地区，调查了病媒的季节性、寄生虫DNA的存在情况以及促成病媒和寄生虫传播的环境因素。进行了为期一年的每月一次的昆虫学调查。对14个住家周围热点地区和6个住家内区域进行了采样。采用聚合酶链反应（PCR）评估白蛉中 DNA的流行情况。使用零膨胀负二项回归来确定微观和中观尺度环境变量与所采样的三种最常见白蛉物种的出现和丰度之间的关联。总共捕获了3543个物种，在所发现的13个物种中， 是优势物种（71.78%）。 、 、 和 在该地区首次被报告。归一化植被指数（NDVI）、与水的距离、降水量、西向东风、风速、最大和最小相对湿度以及性别是与环境中病媒存在/丰度相关的显著变量。住家周围病媒的存在/丰度与降水量、海拔、最高温度、最小和最大相对湿度、西向东风、风速以及性别有关。全年平均在21%的 中检测到 DNA。病媒丰度集中在城市和城市周边地区，一些样本出现在城市的不同区域，一些地点病媒丰度较高。这种分布表明，在流行期间城市地区人类与寄生虫病媒实际接触的风险与城市周边植被区域有关，然后延伸到城市地区。
