Khan Aftab Ahmed, Siddiqui Adel Zia, Syed Jamaluddin, Noori Shafaq, Alkheraif Abdulaziz Abdullah
Dental Biomaterials Research Chair, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia.
Dental Materials Department, Baqai Dental College, Baqai Medical University, Karachi, Pakistan.
Pak J Pharm Sci. 2016 Sep;29(5 Suppl):1823-1827.
The purpose of this in vitro study was to analyse the absorbance of dye material in conventional glass ionomer cement (GIC) by applying various commercially available surface protecting layers on GIC. 90 disc-shaped specimens were made using brass mold measuring 7mm in diameter and 2mm in thickness. 30 specimens were selected for each week testing having 6 groups (n=5). The groups were: G1 (Control group), G2 (Nail polish coated GIC), G3 (Master bond coated GIC), G4 (Copal varnish coated GIC), G5 (Varnal coated GIC), G6 (Cold mold seal coated GIC). The specimens of each group were immersed in a separate test tube filled with methylene blue dye, and placed in an incubator (37°C±2°C) for 1 week, 2 weeks and 3 weeks' time. After required time period, the specimens were rinsed under distal water for 1 minute and air dried for 1 hour. Next, the specimens of each group were put into new test tubes containing 1ml absolute alcohol and again stored at (37°C±2°C) for 24 hours. Absorbance were recorded in ultravoilet spectrophotometer. Results were analysed by Student t-test and Pearson's correlation. The results suggest that varnal and copal varnish are effective protecting materials with significant difference (P<0.01) after 3 weeks time. Our results conclude that the application of suitable protecting material may lead to longevity of GIC restorative biomaterial in a complexed oral environment.
本体外研究的目的是通过在传统玻璃离子水门汀（GIC）上应用各种市售表面保护层，分析染料材料在GIC中的吸光度。使用直径7mm、厚度2mm的黄铜模具制作了90个圆盘形标本。每周测试选择30个标本，分为6组（n = 5）。分组如下：G1（对照组）、G2（涂有指甲油的GIC）、G3（涂有万能胶的GIC）、G4（涂有紫胶清漆的GIC）、G5（涂有Varnal的GIC）、G6（涂有冷模密封剂的GIC）。将每组标本浸入装有亚甲蓝染料的单独试管中，并置于培养箱（37°C±2°C）中1周、2周和3周。在规定时间后，将标本在去离子水下冲洗1分钟，然后风干1小时。接下来，将每组标本放入含有1ml无水乙醇的新试管中，再次在（37°C±2°C）下储存24小时。在紫外分光光度计中记录吸光度。结果通过学生t检验和皮尔逊相关性分析。结果表明，3周后，Varnal和紫胶清漆是有效的保护材料，差异有统计学意义（P<0.01）。我们的结果得出结论，在复杂的口腔环境中，应用合适的保护材料可能会延长GIC修复生物材料的使用寿命。
