Siddiqui Adel Zia, Sultana Nighat, Mirani Zulfiqar Ali, Alkhureif Abdulaziz Abdullah, Rehan Faisal, Siddiqui Iftikhar Ahmed
Adel Zia Siddiqui, MSc, B.D.S, CHPE Professor, Dept of Dental Material Sciences, Baqai Dental College, Karachi, Pakistan.
Nighat Sultana, Post Doc, Ph.D. Chief Scientific Officer, PCSIR Laboratories Complex, Karachi, Pakistan.
Pak J Med Sci. 2024 Sep;40(8):1808-1812. doi: 10.12669/pjms.40.8.9190.
This laboratory study evaluated the effect of () root extracts and Chlorhexidine Digluconate (CHX) on the antibacterial and surface hardness properties of glass ionomer cement (GIC).
The in vitro experimental study was conducted at the Baqai Institute of Pharmaceutical Sciences of Baqai Medical University, Karachi, Pakistan, from October 2022 to March 2023. There were a total four experimental groups. The first group consisted of ethanol extract (GIC-SPEE) and second group consisted of hexane extract as (GIC-SPHE) both prepared from root respectively, and mixed with liquid of GIC separately. The third group comprised chlorhexidine (GIC-CHX) that was also mixing into liquid portion of GIC and the last group was Control i.e. (cGIC). The GIC samples were prepared by using stainless steel metallic moulds with dimension (5mm x 2mm), following the manufacturer guidelines. Antibacterial activity against was done by disc diffusion test (DDT), and surface hardness test was done by Vickers hardness tester. Statistical analysis was performed using One-Way ANOVA and Tukey's post hoc tests (p<0.05).
The antibacterial activity against reported that the maximum zone of inhibition was obtained at 3 wt% by the GIC-SPEE, when compared with other experimental groups. For surface hardness, the highest mean and standard deviation and significant findings was reported by the group GIC-SPEE.
Considering the outcome of this in vitro study, it can be concluded that the addition of 3 wt% GIC-SPEE increased the surface hardness and antibacterial activity against .
本实验室研究评估了（）根提取物和葡萄糖酸氯己定（CHX）对玻璃离子水门汀（GIC）抗菌性能和表面硬度的影响。
2022年10月至2023年3月在巴基斯坦卡拉奇巴凯医科大学巴凯药学院进行了体外实验研究。总共分为四个实验组。第一组由乙醇提取物（GIC-SPEE）组成，第二组由己烷提取物（GIC-SPHE）组成，二者分别由（）根制备，并分别与GIC液体混合。第三组包含氯己定（GIC-CHX），其也混入GIC的液体部分，最后一组为对照组，即（cGIC）。按照制造商指南，使用尺寸为（5mm×2mm）的不锈钢金属模具制备GIC样品。通过纸片扩散法（DDT）对（）进行抗菌活性测试，通过维氏硬度计进行表面硬度测试。使用单因素方差分析和Tukey事后检验进行统计分析（p<0.05）。
对（）的抗菌活性报告显示，与其他实验组相比，GIC-SPEE在3 wt%时获得了最大抑菌圈。对于表面硬度，GIC-SPEE组报告的平均和标准差最高且有显著结果。
考虑到这项体外研究的结果，可以得出结论，添加3 wt%的GIC-SPEE可提高表面硬度和对（）的抗菌活性。
