Sarzani R, Dessì-Fulgheri P, Paci V M, Espinosa E, Rappelli A
Istituto di Medicina Clinica, Università di Ancona, Italy.
J Endocrinol Invest. 1996 Oct;19(9):581-5. doi: 10.1007/BF03349021.
To characterize natriuretic peptide receptor (NPr) gene expression in human tissues, we cloned portions of the cDNAs codifying for NPr with guanylyl cyclase activity (NPr-A and NPr-B) and without guanylyl cyclase activity (NPr-C). Total RNA was extracted from samples taken at surgery from normal human tissues. NPr-A and NPr-B cDNAs obtained from lung as well as NPr-C cDNA obtained from renal cortex were cloned, characterized, and used for comparative Northern analysis. NPr-A mRNA (approximately 4 kb) was most abundant in adipose tissue (8 patients) independently on the site of sampling, whereas it was approximately 2.5-fold and 5-fold less abundant, respectively, in kidney (either renal cortex or papilla from 3 patients) and adrenal (4 patients), known target tissues of natriuretic peptides. NPr-C mRNAs (approximately 7.7 and 6.8 kb) had a similar tissue distribution but the highest levels were found in renal tissue and only very low expression levels were found in adrenals (approximately 20-fold lower than renal cortex). The ratio of NPrA versus NPr-C mRNA levels were highest in adrenal and lowest in renal tissue. NPr-B mRNA (approximately 4 kb), which encodes the receptor for the C-type natriuretic peptide, had a different and wide tissue distribution, including expression in ileum and liver, with the highest levels in venous and prostatic tissue. These results indicate that, in humans, different patterns of NPr expression with different NPr-A/NPr-C mRNA level ratios, are present in known target tissues of natriuretic peptides. "Non-classic" target tissues, such as the adipose one, maximally expressed NPr-A and also NPr-C, suggesting that natriuretic peptides may have wider functional activities than those previously demonstrated.
为了描述利钠肽受体（NPr）基因在人体组织中的表达情况，我们克隆了编码具有鸟苷酸环化酶活性的NPr（NPr - A和NPr - B）以及不具有鸟苷酸环化酶活性的NPr（NPr - C）的部分cDNA。从正常人组织手术采集的样本中提取总RNA。对从肺中获得的NPr - A和NPr - B cDNA以及从肾皮质获得的NPr - C cDNA进行克隆、鉴定，并用于比较性Northern分析。NPr - A mRNA（约4 kb）在脂肪组织（8例患者）中最为丰富，与取样部位无关，而在肾脏（3例患者的肾皮质或乳头）和肾上腺（4例患者）中，其丰度分别约低2.5倍和5倍，肾脏和肾上腺是已知的利钠肽靶组织。NPr - C mRNAs（约7.7和6.8 kb）具有相似的组织分布，但在肾组织中水平最高，在肾上腺中仅发现极低的表达水平（比肾皮质低约20倍）。NPrA与NPr - C mRNA水平的比值在肾上腺中最高，在肾组织中最低。NPr - B mRNA（约4 kb）编码C型利钠肽受体，具有不同且广泛的组织分布，包括在回肠和肝脏中的表达，在静脉和前列腺组织中水平最高。这些结果表明，在人类中，利钠肽已知靶组织中存在不同的NPr表达模式，具有不同的NPr - A/NPr - C mRNA水平比值。“非经典”靶组织，如脂肪组织，最大程度地表达NPr - A以及NPr - C，这表明利钠肽可能具有比先前证明的更广泛的功能活性。
