用于人Th9细胞分化的极化细胞因子

Polarizing Cytokines for Human Th9 Cell Differentiation.

作者信息

Putheti Prabhakar

机构信息

Department of Medicine, Weill-Cornell Medical College, 525 East, 68th Street, Box 3, New York, NY, 10065, USA.

出版信息

Methods Mol Biol. 2017;1585:73-82. doi: 10.1007/978-1-4939-6877-0_6.

DOI:10.1007/978-1-4939-6877-0_6

PMID:28477188

Abstract

CD4+ T helper (Th) cell subset generation in vivo requires T cell receptor activation and surface CD28 co-stimulation in the presence of one or more cytokines. Similarly, Th cells can be generated in vitro by activating naïve CD4+CD25- T cells with plate bound-anti-CD3 monoclonal antibody (mAb) (pbCD3) and soluble-anti-CD28 mAb (sCD28) in the presence of polarizing recombinant (r) cytokines and anti-cytokine mAbs. In comparison to in vitro CD4+CD25- T cells, memory CD4+CD25-CD45RO+ T cells have been shown to convert to Th9 cells more efficiently. Here, protocol for in vitro generation of human Th9 cells by activating CD4+CD25-CD45RO+ memory T cells with pbCD3 and sCD28 in the presence of polarizing recombinant interleukin-4 (rIL-4) and transforming growth factor (rTGF-β) is described.

摘要

体内CD4 +辅助性T（Th）细胞亚群的产生需要T细胞受体激活以及在一种或多种细胞因子存在的情况下通过表面CD28共刺激。同样，在极化重组（r）细胞因子和抗细胞因子单克隆抗体存在的情况下，用板结合抗CD3单克隆抗体（mAb）（pbCD3）和可溶性抗CD28 mAb（sCD28）激活幼稚CD4 + CD25 - T细胞可在体外产生Th细胞。与体外CD4 + CD25 - T细胞相比，记忆CD4 + CD25 - CD45RO + T细胞已被证明能更有效地转化为Th9细胞。在此，描述了在极化重组白细胞介素-4（rIL-4）和转化生长因子（rTGF-β）存在的情况下，用pbCD3和sCD28激活CD4 + CD25 - CD45RO +记忆T细胞体外产生人Th9细胞的方案。