从全血中测定Th9细胞的频率。

Determining the Frequencies of Th9 Cells from Whole Blood.

作者信息

Rajamanickam Anuradha, Babu Subash

机构信息

National Institutes of Health - International Center for Excellence in Research, National Institute of Research in Tuberculosis (Formerly Tuberculosis Research Center), 1, Mayor Sathyamoorty Road, Chetpet, Chennai, India.

出版信息

Methods Mol Biol. 2017;1585:83-92. doi: 10.1007/978-1-4939-6877-0_7.

DOI:10.1007/978-1-4939-6877-0_7

PMID:28477189

Abstract

Th9 cells are a subset of CD4+ T cells producing the cytokine, IL-9. Th9 cells are increasingly recognized as being important player in allergy, autoimmunity, and antitumor responses. The polarization and expansion of Th9 cells requires the cytokines IL-4, TGF-β. In this chapter, we described the protocol for measuring Th9 frequencies using whole blood.

摘要

Th9细胞是产生细胞因子白细胞介素-9（IL-9）的CD4+T细胞亚群。Th9细胞越来越被认为是过敏、自身免疫和抗肿瘤反应中的重要参与者。Th9细胞的极化和扩增需要细胞因子IL-4、转化生长因子-β（TGF-β）。在本章中，我们描述了使用全血测量Th9细胞频率的方法。