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中期染色体结构通过凝聚素I介导的DNA（解）连环作用而动态维持。

Metaphase chromosome structure is dynamically maintained by condensin I-directed DNA (de)catenation.

作者信息

Piskadlo Ewa, Tavares Alexandra, Oliveira Raquel A

机构信息

Instituto Gulbenkian de Ciência, Oeiras, Portugal.

出版信息

Elife. 2017 May 6;6:e26120. doi: 10.7554/eLife.26120.

DOI:10.7554/eLife.26120
PMID:28477406
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5451211/
Abstract

Mitotic chromosome assembly remains a big mystery in biology. Condensin complexes are pivotal for chromosome architecture yet how they shape mitotic chromatin remains unknown. Using acute inactivation approaches and live-cell imaging in embryos, we dissect the role of condensin I in the maintenance of mitotic chromosome structure with unprecedented temporal resolution. Removal of condensin I from pre-established chromosomes results in rapid disassembly of centromeric regions while most chromatin mass undergoes hyper-compaction. This is accompanied by drastic changes in the degree of sister chromatid intertwines. While wild-type metaphase chromosomes display residual levels of catenations, upon timely removal of condensin I, chromosomes present high levels of Topoisomerase II (TopoII)-dependent re-entanglements, and complete failure in chromosome segregation. TopoII is thus capable of re-intertwining previously separated DNA molecules and condensin I continuously required to counteract this erroneous activity. We propose that maintenance of chromosome resolution is a highly dynamic bidirectional process.

摘要

有丝分裂染色体组装仍是生物学中的一个重大谜团。凝聚素复合物对染色体结构至关重要，但它们如何塑造有丝分裂染色质仍不清楚。我们利用胚胎中的急性失活方法和活细胞成像技术，以前所未有的时间分辨率剖析了凝聚素I在维持有丝分裂染色体结构中的作用。从预先形成的染色体中去除凝聚素I会导致着丝粒区域迅速解体，而大多数染色质质量会经历超浓缩。这伴随着姐妹染色单体缠绕程度的剧烈变化。野生型中期染色体显示出残留水平的连环结构，而在及时去除凝聚素I后，染色体呈现出高水平的依赖拓扑异构酶II（TopoII）的重新缠绕，并且染色体分离完全失败。因此，TopoII能够重新缠绕先前分离的DNA分子，而凝聚素I则持续需要抵消这种错误活动。我们提出，维持染色体分辨率是一个高度动态的双向过程。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a0/5451211/a1ce37b4185a/elife-26120-fig6-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a0/5451211/6f70105a7d46/elife-26120-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a0/5451211/acae168a76f2/elife-26120-fig1-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a0/5451211/570ee43ae363/elife-26120-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a0/5451211/5c5b8adfa376/elife-26120-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a0/5451211/c94a314f423f/elife-26120-fig3-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a0/5451211/4ec273630b6c/elife-26120-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a0/5451211/a8c9f032c0c1/elife-26120-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a0/5451211/57e1e3a6fa53/elife-26120-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a0/5451211/a1ce37b4185a/elife-26120-fig6-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a0/5451211/6f70105a7d46/elife-26120-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a0/5451211/acae168a76f2/elife-26120-fig1-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a0/5451211/570ee43ae363/elife-26120-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a0/5451211/5c5b8adfa376/elife-26120-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a0/5451211/c94a314f423f/elife-26120-fig3-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a0/5451211/4ec273630b6c/elife-26120-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a0/5451211/a8c9f032c0c1/elife-26120-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a0/5451211/57e1e3a6fa53/elife-26120-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a0/5451211/a1ce37b4185a/elife-26120-fig6-figsupp1.jpg

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