Dewanjee Saikat, Joardar Swarnalata, Bhattacharjee Niloy, Dua Tarun K, Das Subhadip, Kalita Jatin, Manna Prasenjit
Advanced Pharmacognosy Research Laboratory, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, India.
Advanced Pharmacognosy Research Laboratory, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, India.
Food Chem Toxicol. 2017 Jul;105:322-336. doi: 10.1016/j.fct.2017.05.002. Epub 2017 May 3.
Ipomoea aquatica Forssk. (Convolvulaceae) is an aquatic vegetable traditionally employed against toxic effects of xenobiotics. The present study has been designed to investigate the molecular mechanism underlying the beneficial role of the edible (aqueous) leaf extract of I. aquatica (AEIA) against doxorubicin (Dox)-induced liver injury. AEIA exhibited a dose-dependent (∼400 μg/ml) increase in cell viability against Dox (1 μM) in isolated rodent hepatocytes. AEIA (400 μg/ml) prevented the Dox-induced increase in ROS, redox imbalance, and activation of mitogen activated protein kinases (MAPK) and intrinsic pathway of apoptosis in hepatocytes. In the in vivo assay, administration of AEIA (100 mg/kg, p.o.) against Dox (3 mg/kg, i.p.) also reduced the oxidative impairment, DNA fragmentation, ATP formation, and up-regulated the mitochondrial co-enzymes Qs in the liver tissues of Wistar rats. Histological assessments were in agreement with the biochemical findings. Substantial quantities of phyto-antioxidants in AEIA may mediate its beneficial function against Dox-induced liver injury.
蕹菜（旋花科）是一种传统上用于对抗异生物质毒性作用的水生蔬菜。本研究旨在探究蕹菜可食用（水提）叶提取物（AEIA）对阿霉素（Dox）诱导的肝损伤发挥有益作用的分子机制。在分离的啮齿动物肝细胞中，AEIA对1 μM的Dox表现出剂量依赖性（约400 μg/ml）的细胞活力增加。AEIA（400 μg/ml）可防止Dox诱导的肝细胞中活性氧增加、氧化还原失衡、丝裂原活化蛋白激酶（MAPK）激活以及凋亡的内在途径。在体内试验中，给Wistar大鼠灌胃AEIA（100 mg/kg）对抗腹腔注射的Dox（3 mg/kg）也可减轻氧化损伤、DNA片段化、ATP生成，并上调肝脏组织中的线粒体辅酶Q。组织学评估与生化结果一致。AEIA中大量的植物抗氧化剂可能介导其对Dox诱导的肝损伤的有益作用。
