Sabahi S, Fekrat L, Zakiaghl M, Moravej G H
Dept of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
Neotrop Entomol. 2018 Feb;47(1):96-105. doi: 10.1007/s13744-017-0522-2. Epub 2017 May 6.
Nowadays, with increasing trend of trans-boundary transportation of agricultural products and higher probability of introduction of many invasive species into new areas, fast and precise species diagnosis is of great significance particularly at the port of entry, where morphological identification often requires adult insect specimens especially with specialist insects. The cucumber fruit fly, Dacus ciliatus Loew (Diptera: Tephritidae), ranks as one of the most destructive agricultural pests attacking mainly fruits of Cucurbitaceae. This pest is also widespread and highly invasive; thus, it is a high priority for pest detection and quarantine programs. Although cucumber fruit fly adults can usually be identified and distinguished from the other species by morphological keys, it is often difficult or impossible to distinguish this species from the other tephritids that share host plants by using material from other stages of development. In such situations, using a quick and robust alternative species diagnostic tool would be valuable. In this study, we assessed a technique combining loop-mediated isothermal amplification (LAMP) with PCR (PCR-LAMP) for the rapid detection and discrimination of cucumber fruit fly DNA from some other common tephritid species attacking Cucurbitaceae, using material from different stages of development. The described method was species-specific and sensitive and provided a rapid diagnostic tool to detect D. ciliaus even by non-experts.
如今，随着农产品跨境运输趋势的增加以及许多入侵物种被引入新区域的可能性提高，快速准确的物种诊断具有重要意义，特别是在入境口岸，形态学鉴定通常需要成虫标本，尤其是对于专业性昆虫。黄瓜实蝇，Dacus ciliatus Loew（双翅目：实蝇科），是攻击葫芦科果实的最具破坏性的农业害虫之一。这种害虫分布广泛且极具入侵性；因此，它是害虫检测和检疫计划的高度优先事项。虽然黄瓜实蝇成虫通常可以通过形态学特征与其他物种区分开来，但使用发育其他阶段的材料往往很难或无法将该物种与其他共享寄主植物的实蝇科昆虫区分开来。在这种情况下，使用一种快速且可靠的替代物种诊断工具将很有价值。在本研究中，我们评估了一种将环介导等温扩增（LAMP）与PCR（PCR-LAMP）相结合的技术，用于使用不同发育阶段的材料，从其他一些攻击葫芦科的常见实蝇科物种中快速检测和鉴别黄瓜实蝇的DNA。所描述的方法具有物种特异性且灵敏，即使非专业人员也能提供一种快速诊断工具来检测黄瓜实蝇。
