Khatun Marina, Habib M Rowshanul, Rabbi M Ahasanur, Amin Ruhul, Islam M Farhadul, Nurujjaman M, Karim M Rezaul, Rahman M Habibur
Department of Biochemistry and Molecular Biology, Rajshahi University, Rajshahi 6205, Bangladesh.
Bangladesh Council of Scientific & Industrial Research (BCSIR) Laboratories, Rajshahi, Bangladesh.
Exp Toxicol Pathol. 2017 Sep 5;69(7):469-476. doi: 10.1016/j.etp.2017.03.008. Epub 2017 May 3.
For scientific clarification of some traditional uses, this study was designed to explore the antioxidant, cytotoxic and antineoplastic properties of leaf extract of Carissa carandas Linn., a traditional medicinal plant of Bangladesh. The methanol extract of Carissa carandas leaves (MELC) was applied on DPPH and ABTS experiments to determine its antioxidant activity. In vitro the cytotoxic effect of MELC was evaluated against colonic adenocarcinoma cell lines (SW-480 and SW-48) whereas in vivo its antineoplastic property was tested against Ehrlich ascites carcinoma (EAC). The DPPH and ABTS assays revealed the antioxidant activity of MELC with IC 10.5±1.2 and 1.75±0.3μg/ml that was comparable to L-ascorbic acid. In vitro cytotoxic study, MELC reduced the viability of adenocarcinoma cells in dose dependent manner and in vivo, administration of MELC (25mg/kg) resulted in a significant (p<0.05) decrease in viable EAC cell count thereby increasing the life span of the EAC cell bearing mice. Restoration of hematological parameters such as red blood cells (RBC), hemoglobin and white blood cells (WBC) to normal levels in MELC-treated mice was also observed. Moreover, treatment with MELC induced apoptosis of EAC cells as observed in fluorescence microscopic view of DAPI (4,6-diamidino-2-phenylindole) stained cells and also increased p53 gene expression MELC-treated cells in respect to untreated EAC control. In addition, the MELC was rich in polyphenol content and its GC-MS chromatogram confirmed the presence of some compounds all of which showed anticancer and cytotoxic activities in previous studies. In a word, this study supports the use of Carissa carandas in traditional medicine as well as highlights the need to further explore the potentials of MELC as an antineoplastic agent.
为了从科学角度阐明某些传统用途，本研究旨在探究孟加拉国传统药用植物大花假虎刺叶提取物的抗氧化、细胞毒性和抗肿瘤特性。大花假虎刺叶的甲醇提取物（MELC）用于二苯基苦味酰基自由基（DPPH）和2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）实验，以测定其抗氧化活性。体外实验中，评估了MELC对结肠腺癌细胞系（SW-480和SW-48）的细胞毒性作用，而在体内实验中，测试了其对艾氏腹水癌（EAC）的抗肿瘤特性。DPPH和ABTS分析显示，MELC的抗氧化活性IC50为10.5±1.2和1.75±0.3μg/ml，与L-抗坏血酸相当。体外细胞毒性研究表明，MELC以剂量依赖性方式降低腺癌细胞的活力，在体内，给予MELC（25mg/kg）可使存活的EAC细胞数量显著减少（p<0.05），从而延长携带EAC细胞小鼠的寿命。还观察到，经MELC处理的小鼠血液学参数如红细胞（RBC）、血红蛋白和白细胞（WBC）恢复到正常水平。此外，在4',6-二脒基-2-苯基吲哚（DAPI）染色细胞的荧光显微镜观察中，发现用MELC处理可诱导EAC细胞凋亡，并且与未处理的EAC对照相比，MELC处理的细胞中p53基因表达也增加。此外，MELC富含多酚，其气相色谱-质谱联用（GC-MS）色谱图证实了某些化合物的存在，所有这些化合物在先前的研究中均显示出抗癌和细胞毒性活性。总之，本研究支持大花假虎刺在传统医学中的应用，并强调需要进一步探索MELC作为抗肿瘤药物的潜力。
