苏云金芽孢杆菌菌株BM-BT15426的全基因组序列及生物信息学分析
Complete genome sequence and bioinformatics analyses of Bacillus thuringiensis strain BM-BT15426.
作者信息
Liu Junyan, Li Lin, Peters Brian M, Li Bing, Chen Dingqiang, Xu Zhenbo, Shirtliff Mark E
机构信息
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, PR China; Department of Clinical Pharmacy, College of Pharmacy, University of Tennessee Health Science Center, Memphis TN 38163, USA.
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, PR China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, PR China.
出版信息
Microb Pathog. 2017 Jul;108:55-60. doi: 10.1016/j.micpath.2017.05.006. Epub 2017 May 4.
OBJECTIVES
This study aimed to investigate the genetic characteristics of Bacillus thuringiensis strain BM-BT15426.
METHODS
B. thuringiensis strain was identified by sequencing the PCR product (amplifying 16S rRNA gene) using ABI Prism 377 DNA Sequencer. The genome was sequenced using PacBio RS II sequencers and assembled de novo using HGAP. Also, further genome annotation was performed.
RESULTS
The genome of B. thuringiensis strain BM-BT15426 has a length of 5,246,329 bp and contains 5409 predicted genes with an average G + C content of 35.40%. Three genes were involved in the "Infectious diseases: Amoebiasis" pathway. A total of 21 virulence factors and 9 antibiotic resistant genes were identified.
CONCLUSIONS
The major pathogenic factors of B. thuringiensis strain BM-BT15426 were identified through complete genome sequencing and bioinformatics analyses which contributes to further study on pathogenic mechanism and phenotype of B. thuringiensis.
目的
本研究旨在调查苏云金芽孢杆菌菌株BM - BT15426的遗传特征。
方法
使用ABI Prism 377 DNA测序仪对PCR产物（扩增16S rRNA基因）进行测序，以鉴定苏云金芽孢杆菌菌株。使用PacBio RS II测序仪对基因组进行测序，并使用HGAP进行从头组装。此外，还进行了进一步的基因组注释。
结果
苏云金芽孢杆菌菌株BM - BT15426的基因组长度为5,246,329 bp，包含5409个预测基因，平均G + C含量为35.40%。有三个基因参与“传染病：阿米巴病”途径。共鉴定出21个毒力因子和9个抗生素抗性基因。
结论
通过全基因组测序和生物信息学分析确定了苏云金芽孢杆菌菌株BM - BT15426的主要致病因素，这有助于进一步研究苏云金芽孢杆菌的致病机制和表型。
Zotero 插件