Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do, 24341, Republic of Korea.
Department of Medical Biomaterials Engineering and Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, Republic of Korea.
Microb Pathog. 2019 Jan;126:14-18. doi: 10.1016/j.micpath.2018.10.013. Epub 2018 Oct 13.
Bacillus thuringiensis promotes the growth of numerous economically important crops. The present study presents the complete genome sequence for a mega plasmid present in the type strain of B. thuringiensis ATCC 10792, a typical spore-forming Gram-positive bacterium with insecticidal activity, and investigates its genetic characteristics. The genome was sequenced and assembled de novo using Pac-Bio sequencers and the Hierarchical Genome Assembly Process, respectively. Further genome annotation was performed, and a total of 489 proteins and a novel mega-plasmid (poh1) with 584,623 bps were identified. The organization of poh1 revealed the genes involved in the insecticidal toxin pathway. The genes responsible for antimicrobial, insecticidal and antibiotic activities were well conserved in poh1, indicating an intimate association with plant hosts. The poh1 plasmid contains the gene encoding a novel crystal protein kinase responsible for production of zeta toxin, which poisons insects and other Gram-negative bacteria through the global inhibition of peptidoglycan synthesis. Lantibiotics are a group of bacteriocins that include the biologically active antimicrobial peptide Paenibacillin. Further, poh1 also contains the genes that encode the gramicidin S prototypical antibiotic peptide and tetracycline resistance protein. In conclusion, the strain-specific genes of B. thuringiensis strain ATCC 10792 were identified through complete genome sequencing and bioinformatics data based on major pathogenic factors that contribute to further studies of the pathogenic mechanism and phenotype analyses.
苏云金芽孢杆菌促进了许多重要经济作物的生长。本研究呈现了存在于苏云金芽孢杆菌 ATCC 10792 典型芽孢形成革兰氏阳性细菌的杀虫活性的型菌株中的巨型质体的完整基因组序列，并研究了其遗传特征。使用 PacBio 测序仪和分级基因组组装流程分别对基因组进行了测序和从头组装。进一步进行了基因组注释，总共鉴定出 489 种蛋白质和一种新型巨型质粒（poh1），大小为 584623bp。poh1 的组织揭示了参与杀虫毒素途径的基因。poh1 中很好地保守了对抗微生物、杀虫和抗生素活性的基因，表明与植物宿主之间存在密切的关联。poh1 质粒包含编码一种新型晶体蛋白激酶的基因，该基因负责产生 zeta 毒素，通过全球抑制肽聚糖合成来毒害昆虫和其他革兰氏阴性细菌。类细菌素是一组包括生物活性抗菌肽 Paenibacillin 的细菌素。此外，poh1 还包含编码革兰氏菌素 S 原型抗生素肽和四环素抗性蛋白的基因。总之，通过完整基因组测序和基于主要致病因子的生物信息学数据鉴定了苏云金芽孢杆菌菌株 ATCC 10792 的菌株特异性基因，这些基因有助于进一步研究致病机制和表型分析。
