多重耐药铜绿假单胞菌 Guangzhou-Pae617 的全基因组分析，其携带巨型质粒 pBM413。
Complete genomic analysis of multidrug-resistance Pseudomonas aeruginosa Guangzhou-Pae617, the host of megaplasmid pBM413.
机构信息
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, PR China; Department of Clinical Pharmacy, College of Pharmacy, University of Tennessee Health Science Center, Memphis TN 38163, USA.
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, PR China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, PR China.
出版信息
Microb Pathog. 2018 Apr;117:265-269. doi: 10.1016/j.micpath.2018.02.049. Epub 2018 Feb 24.
OBJECTIVES
We previously described the novel qnrVC6 and bla carrying megaplasmid pBM413. This study aimed to investigate the complete genome of multidrug-resistance P. aeruginosa Guangzhou-Pae617, a clinical isolate from the sputum of a patient who was suffering from respiratory disease in Guangzhou, China.
METHODS
The genome was sequenced using Illumina Hiseq 2500 and PacBio RS II sequencers and assembled de novo using HGAP. The genome was automatically and manually annotated.
RESULTS
The genome of P. aeruginosa Guangzhou-Pae617 is 6,430,493 bp containing 5881 predicted genes with an average G + C content of 66.43%. The genome showed high similarity to two new sequenced P. aeruginosa strains isolated from New York, USA. From the whole genome sequence, we identified a type IV pilin, two large prophages, 15 antibiotic resistant genes, 5 genes involved in the "Infectious diseases" pathways, and 335 virulence factors.
CONCLUSIONS
The antibiotic resistance and virulence factors in the genome of P. aeruginosa strain Guangzhou-Pae617 were identified by complete genomic analysis. It contributes to further study on antibiotic resistance mechanism and clinical control of P. aeruginosa.
目的
我们之前描述了新型 qnrVC6 和 bla 携带的巨型质粒 pBM413。本研究旨在对来自中国广州一名患有呼吸道疾病患者痰液中分离的多药耐药性铜绿假单胞菌 Guangzhou-Pae617 的完整基因组进行研究。
方法
使用 Illumina Hiseq 2500 和 PacBio RS II 测序仪对基因组进行测序，并使用 HGAP 从头组装。基因组采用自动和手动注释。
结果
铜绿假单胞菌 Guangzhou-Pae617 的基因组大小为 6430493bp，包含 5881 个预测基因，平均 G+C 含量为 66.43%。该基因组与从美国纽约分离的两个新测序的铜绿假单胞菌菌株高度相似。从全基因组序列中，我们鉴定了一种 IV 型菌毛、两个大型噬菌体、15 个抗生素耐药基因、5 个参与“传染病”途径的基因和 335 个毒力因子。
结论
通过完整的基因组分析，鉴定了铜绿假单胞菌 Guangzhou-Pae617 基因组中的抗生素耐药和毒力因子。这有助于进一步研究铜绿假单胞菌的抗生素耐药机制和临床控制。
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