Padmapriya Ramamoorthy, Gayathri Loganathan, Ronsard Larance, Akbarsha Mohammad A, Raveendran Ramasamy
Department of Pharmacology, JIPMER, Puducherry, India.
Mahatma Gandhi-Doerenkamp Center, Bharathidasan University, Tiruchirappalli, India.
Pharmacogn Mag. 2017 Jan;13(Suppl 1):S16-S21. doi: 10.4103/0973-1296.203981. Epub 2017 Apr 7.
is an Indian herb used in traditional medicine to treat various diseases such as jaundice, asthma, liver and urinary disorders. However, the anti-cancer potential of on hepatocellular carcinoma (HCC) is poorly understood. Therefore, this study aims to investigate the anti-cancer activity of in HepG2 hepatocellular carcinoma cells.
The leaves and root of were extracted with methanol using soxhlet apparatus. The cytotoxicity of the extracts in HepG2 cells was evaluated using MTT assay whereas the mode of cell death was examined by AOEB, Hoechst and JC1 staining under a fluorescence microscope. extracts-induced caspase-3 expression was investigated using colorimetric assay.
The leaves and root extracts inhibited HepG2 cell growth at the IC of 102.33 ± 10.26 µg/mL and 276.67 ± 20.43 µg/mL respectively at 24 h. Chromatin condensation, nuclear fragmentation, apoptotic bodies formation and mitochondrial membrane depolarization were observed in HepG2 cells treated with both extracts. The caspase-3 expression was significantly ( < 0.05) increased in extracts treated cells when compared to control.
The leaves and root extracts of induce apoptosis mediated cell death in HepG2 cells.
The leaves and root extracts of exhibited anticancer activity in HepG2 hepatocellular carcinoma cells. These extracts induced cell shrinkage, DNA condensation and fragmentation, mitochondrial membrane depolarization and upregulated caspase-3 expression indicating extracts induce apoptosis in HepG2 cells. AO: acridine orange, DMSO: dimethyl sulfoxide, EB: ethidium bromide, IC50: the concentration at which 50% of cancer cells are dead, JC-1: 5, 5', 6, 6'-tetrachloro-1, 1', 3, 3'-tetraethyl-imidacarbocyanine iodide, MTT: 3-4, 5-dimethylthiazole-2-yl, 2,5-diphenyl tetrazolium bromide, PBS: phosphate-buffered saline, ΔΨm: mitochondrial trans-membrane potential.
[某种植物名称]是一种印度草药，在传统医学中用于治疗各种疾病，如黄疸、哮喘、肝脏和泌尿系统疾病。然而，其对肝细胞癌（HCC）的抗癌潜力了解甚少。因此，本研究旨在探讨[某种植物名称]对HepG2肝癌细胞的抗癌活性。
使用索氏提取器用甲醇提取[某种植物名称]的叶和根。使用MTT法评估[某种植物名称]提取物对HepG2细胞的细胞毒性，而在荧光显微镜下通过吖啶橙-溴化乙锭（AOEB）、Hoechst和JC1染色检查细胞死亡方式。使用比色法研究[某种植物名称]提取物诱导的半胱天冬酶-3表达。
叶和根提取物在24小时时分别以102.33±10.26μg/mL和276.67±20.43μg/mL的半数抑制浓度（IC50）抑制HepG2细胞生长。在用两种提取物处理的HepG2细胞中观察到染色质浓缩、核碎裂、凋亡小体形成和线粒体膜去极化。与对照相比，提取物处理的细胞中半胱天冬酶-3表达显著（P<0.05）增加。
[某种植物名称]的叶和根提取物在HepG2细胞中诱导凋亡介导的细胞死亡。
[某种植物名称]的叶和根提取物在HepG2肝癌细胞中表现出抗癌活性。这些提取物诱导细胞收缩、DNA浓缩和片段化、线粒体膜去极化并上调半胱天冬酶-3表达，表明[某种植物名称]提取物在HepG2细胞中诱导凋亡。AO：吖啶橙，DMSO：二甲基亚砜，EB：溴化乙锭，IC50：50%癌细胞死亡时的浓度，JC-1：5,5',6,6'-四氯-1,1',3,3'-四乙基-咪唑羰花青碘化物，MTT：3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐，PBS：磷酸盐缓冲盐水，ΔΨm：线粒体跨膜电位 。
