Moselhy Said S, Ghoneim Magdy A, Khan Jehan A
Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
Bioactive Natural Products Research Group, KAU.
Afr J Tradit Complement Altern Med. 2016 Sep 29;13(6):18-21. doi: 10.21010/ajtcam.v13i6.4. eCollection 2016.
The current trend globally is the utilization of natural products as therapeutic agents given its minimum side effects. The leaves of contain several active ingredient compounds such as rebaudioside. extract have been used for many purposes. Active oxygen radicals can induce base modifications, DNA breakage, and intracellular protein crosslink's. This study was done to evaluate the potential of extract as antibacterial and antioxidants actions.
Antibacterial activity of different extracts of was tested in vitro against different species of bacteria and hepato-protective efficacy was testes in rats injected with CCl as hepatotoxic.
Acetone extract exhibited antibacterial activity against selected five bacteria species. The acetone extract suppressed the elevation of serum ALT (p <0.05) and AST (p <0.001) activities induced by CCl. Animals given stevia extract showed prevention against deleterious effects of CCl by lowering lipid peroxidation and enhancement of antioxidant activities as SOD and CAT. The protection trial is better than treatment trial. Total phenolic content of aqueous and acetone extracts were found 30 mg and 85 mg gallic /gm extract respectively. While the total flavonoids were 40 mg and 80 mg quercetin/g respectively. The GC-MS analysis showed that monoterpene and indole are the main components. Aqueous extract don't show any antibacterial activity against the tested strains. The antioxidant properties were attributable to its phenolic content to scavenge free radicals.
Acetone extract possess a potent antimicrobial and activity against deleterious effect of CCl-caused liver damage.
鉴于天然产物副作用最小，全球目前的趋势是将其用作治疗剂。甜叶菊的叶子含有多种活性成分化合物，如莱鲍迪甙。甜叶菊提取物已被用于多种用途。活性氧自由基可诱导碱基修饰、DNA断裂和细胞内蛋白质交联。本研究旨在评估甜叶菊提取物的抗菌和抗氧化作用潜力。
测试了甜叶菊不同提取物对不同细菌种类的体外抗菌活性，并在注射四氯化碳作为肝毒素的大鼠中测试了其肝脏保护功效。
丙酮提取物对所选的五种细菌表现出抗菌活性。丙酮提取物抑制了四氯化碳诱导的血清谷丙转氨酶（p<0.05）和谷草转氨酶（p<0.001）活性的升高。给予甜叶菊提取物的动物通过降低脂质过氧化和增强超氧化物歧化酶（SOD）和过氧化氢酶（CAT）等抗氧化活性，显示出对四氯化碳有害影响的预防作用。保护试验优于治疗试验。水提取物和丙酮提取物的总酚含量分别为30毫克和85毫克没食子酸/克提取物。而总黄酮含量分别为40毫克和80毫克槲皮素/克。气相色谱-质谱分析表明，单萜和吲哚是主要成分。水提取物对测试菌株没有显示出任何抗菌活性。其抗氧化特性归因于其酚类成分对自由基的清除作用。
丙酮提取物具有强大的抗菌活性以及对四氯化碳所致肝损伤有害影响的活性。
