Graduate School of Environmental Studies, Tohoku University , Sendai, Miyagi 980-8576, Japan.
Division of Electrical Engineering and Computer Science, Kakuma-machi, Kanazawa University , Kanazawa 920-1192, Japan.
Anal Chem. 2017 Jun 6;89(11):6015-6020. doi: 10.1021/acs.analchem.7b00584. Epub 2017 May 12.
Observation of nanoscale structure dynamics on cell surfaces is essential to understanding cell functions. Hopping-mode scanning ion conductance microscopy (SICM) was used to visualize the topography of fragile convoluted nanoscale structures on cell surfaces under noninvasive conditions. However, conventional hopping mode SICM does not have sufficient temporal resolution to observe cell-surface dynamics in situ because of the additional time required for performing vertical probe movements of the nanopipette. Here, we introduce a new scanning algorithm for high speed SICM measurements using low capacitance and high-resonance-frequency piezo stages. As a result, a topographic image is taken within 18 s with a 64 × 64 pixel resolution at 10 × 10 μm. The high speed SICM is applied to the characterization of microvilli dynamics on surfaces, which shows clear structural changes after the epidermal growth factor stimulation.
观察细胞表面的纳米级结构动力学对于理解细胞功能至关重要。跳跃模式扫描离子电导显微镜（SICM）被用于在非侵入条件下可视化细胞表面易碎卷曲纳米级结构的形貌。然而，由于纳米管进行垂直探针运动所需的额外时间，传统的跳跃模式 SICM 没有足够的时间分辨率来原位观察细胞表面动力学。在这里，我们引入了一种新的扫描算法，用于使用低电容和高共振频率的压电台进行高速 SICM 测量。结果，以 64×64 像素的分辨率在 10×10 μm 的范围内以 18 s 的速度拍摄了一张形貌图像。高速 SICM 被应用于表面微绒毛动力学的表征，在表皮生长因子刺激后显示出明显的结构变化。
