Institute of Applied Physics, University of Tübingen, Auf der Morgenstelle 10, 72076 Tübingen, Germany.
Nanoscale. 2019 Apr 25;11(17):8579-8587. doi: 10.1039/c8nr10162k.
Scanning ion conductance microscopy (SICM) is an emerging tool for non-invasive and high-resolution topography imaging of live cells. However, the imaging speed of conventional SICM setups is slow, requiring several seconds or even minutes per image, thereby making it difficult to study cellular dynamics. Here, we describe a high-speed SICM (HS-SICM) setup for topography imaging in the hopping mode with a pixel rate of 11.0 kHz, which is 15 times faster than what was reported before. In combination with a "turn step" procedure for rapid pipette retraction, we image the ultra-fast morphodynamics of live human platelets, A6 cells, and U2OS cells at a rate as fast as 0.6 s per frame. The results show that HS-SICM provides a useful platform for investigating the dynamics of cell morphology on a sub-second timescale.
扫描离子电导显微镜（SICM）是一种新兴的工具，可用于对活细胞进行非侵入式和高分辨率形貌成像。然而，传统 SICM 设备的成像速度较慢，每张图像需要几秒钟甚至几分钟的时间，因此很难研究细胞动力学。在这里，我们描述了一种用于跳跃模式下形貌成像的高速 SICM（HS-SICM）设置，其像素率为 11.0 kHz，比之前报道的速度快 15 倍。结合用于快速缩回探头的“转角步”程序，我们以高达 0.6 s/帧的速度对活的人血小板、A6 细胞和 U2OS 细胞的超快形态动力学进行成像。结果表明，HS-SICM 为研究细胞形态的动力学提供了一个有用的平台，其时间尺度可达到亚秒级。
