Ihssen Julian, Jankowska Dagmara, Ramsauer Thomas, Reiss Renate, Luchsinger Ronny, Wiesli Luzia, Schubert Mark, Thöny-Meyer Linda, Faccio Greta
Laboratory for Biomaterials, Empa, Swiss Federal Laboratories for Materials Science and Technology, Lerchenfeldstrasse 5, 9014 St. Gallen, CH, Switzerland.
Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science and Technology, Lerchenfeldstrasse 5, 9014 St. Gallen, CH, Switzerland.
Protein Eng Des Sel. 2017 Jun 1;30(6):449-453. doi: 10.1093/protein/gzx026.
Laccases and laccase-like multi-copper oxidases (LMCOs) are versatile and robust biocatalysts applied in a variety of oxidative processes, and various studies have attempted to improve their catalytic activity. Here we report the engineering of a bacterial LMCO for enhanced oxidation of the lignin-related compound guaiacol by a combination of structure-guided mutagenesis and DNA shuffling. Mutant L9 showed a 1.39 mM Km for guaiacol and a 2.5-fold increase in turnover rate (kcat/Km = 2.85·104 M-1s-1).
漆酶和漆酶样多铜氧化酶（LMCOs）是应用于各种氧化过程的多功能且强大的生物催化剂，并且各种研究已尝试提高它们的催化活性。在此，我们报告了通过结构引导诱变和DNA改组相结合的方法对一种细菌LMCO进行工程改造，以增强其对木质素相关化合物愈创木酚的氧化作用。突变体L9对愈创木酚的米氏常数（Km）为1.39 mM，周转速率提高了2.5倍（kcat/Km = 2.85·104 M-1s-1）。
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