Chauhan Prakram Singh, Goradia Bindi, Saxena Arunika
School of Biological Sciences, G. B. Pant, University of Agricultural and Technology, Pantnagar, Uttarakhand 263145 India.
Marine Biotechnology and Ecology Division, Council of Scientific and Industrial Research - Central Salt & Marine Chemicals Research Institute (CSIR-CSMCRI), Bhavnagar, Gujarat 364 021 India.
3 Biotech. 2017 Oct;7(5):323. doi: 10.1007/s13205-017-0955-7. Epub 2017 Sep 16.
Laccases (benzenediol: oxygen oxidoreductase, EC 1.10.3.2) are multi-copper enzymes which catalyze the oxidation of a wide range of phenolic and non-phenolic aromatic compounds in the presence or absence of a mediator. Till date, laccases have mostly been isolated from fungi and plants, whereas laccase from bacteria has not been well studied. Bacterial laccases have several unique properties that are not characteristics of fungal laccases such as stability at high temperature and high pH. Bacteria produce these enzymes either extracellularly or intracellularly and their activity is in a wide range of temperature and pH. It has application in pulp biobleaching, bioremediation, textile dye decolorization, pollutant degradation, biosensors, etc. Hence, comprehensive information including sources, production conditions, characterization, cloning and biotechnological applications is needed for the effective understanding and application of these enzymes at the industrial level. The present review provides exhaustive information of bacterial laccases reported till date.
漆酶(苯二酚:氧氧化还原酶,EC 1.10.3.2)是一种多铜酶,在有或没有介体的情况下,能催化多种酚类和非酚类芳香族化合物的氧化反应。迄今为止,漆酶大多是从真菌和植物中分离得到的,而细菌来源的漆酶尚未得到充分研究。细菌漆酶具有一些真菌漆酶所不具备的独特性质,如在高温和高pH条件下的稳定性。细菌产生这些酶的方式有胞外分泌和胞内合成两种,其活性在较宽的温度和pH范围内都能保持。漆酶在纸浆生物漂白、生物修复、纺织染料脱色、污染物降解、生物传感器等领域都有应用。因此,为了在工业层面有效理解和应用这些酶,需要包括来源、生产条件、特性、克隆及生物技术应用等方面的全面信息。本综述提供了迄今为止已报道的细菌漆酶的详尽信息。