Macartney L, McCandlish I A, Thompson H, Cornwell H J
Department of Pathology, Glasgow Veterinary School.
Res Vet Sci. 1988 Sep;45(2):170-3. doi: 10.1016/S0034-5288(18)30926-3.
Two techniques, adsorption on to hydroxylapatite and density gradient centrifugation, were investigated as prospective methods for the large scale purification of canine parvovirus from faecal suspensions. Adsorption with hydroxylapatite successfully removed virus from faecal material. However, the resultant virus was contaminated and some virus was left behind in the faecal suspension. Repeated adsorption with hydroxylapatite appeared to result in some damage to the virus particles. In contrast, density gradient centrifugation provided a simple, economical method of purification which yielded uncontaminated, infectious virus. The final method, using both isopyknic and rate zonal centrifugation is described.
研究了两种技术，即羟基磷灰石吸附法和密度梯度离心法，作为从粪便悬液中大规模纯化犬细小病毒的潜在方法。用羟基磷灰石吸附可成功从粪便物质中去除病毒。然而，所得病毒受到污染，且粪便悬液中仍残留一些病毒。用羟基磷灰石反复吸附似乎对病毒颗粒造成了一些损害。相比之下，密度梯度离心提供了一种简单、经济的纯化方法，可产生未受污染的感染性病毒。本文描述了最终使用等密度离心和速率区带离心的方法。
