Yasuhara H, Matsui O, Hirahara T, Ohgitani T, Tanaka M, Kodama K, Nakai M, Sasaki N
Nihon Juigaku Zasshi. 1989 Apr;51(2):337-44. doi: 10.1292/jvms1939.51.337.
A small DNA virus was isolated from the feces of a sow with diarrhea and identified as a parvovirus on the basis of its properties. The virus replicated preferentially in cell cultures of swine origin, including primary porcine thyroid gland and kidney cell cultures in which the cytopathic effect developed. The virus agglutinated erythrocytes of guinea pig, mouse and human group O but not these of chicken. The growth of the virus was inhibited by 5-iodo-2'-deoxyuridine. The virus was resistant to ether and heating at 56 degrees C for 30 min and stable at pH 3.0. The buoyant density of the infectious particles was 1.40 g/ml in CsCl density gradient, and the virions were 27 nm in diameter by electron microscopy. The viral protein seemed to be separated into four polypeptides with molecular weights of 81k, 70k, 66k and 62k daltons respectively. Cross serum neutralization test demonstrated that the virus was antigenically different from porcine parvovirus as well as bovine and canine parvoviruses. These findings and the survey on neutralizing antibody distribution indicated indirectly that another parvovirus which could be antigenically distinguished from well-known porcine parvovirus had been widespread among swine in Japan.
从一头腹泻母猪的粪便中分离出一种小型DNA病毒，并根据其特性鉴定为细小病毒。该病毒优先在猪源细胞培养物中复制，包括原代猪甲状腺和肾细胞培养物，在这些培养物中会产生细胞病变效应。该病毒能凝集豚鼠、小鼠和人类O型血红细胞，但不能凝集鸡的红细胞。5-碘-2'-脱氧尿苷可抑制该病毒的生长。该病毒对乙醚有抗性，在56℃加热30分钟仍稳定，在pH 3.0时也稳定。在氯化铯密度梯度中，感染性颗粒的浮力密度为1.40 g/ml，通过电子显微镜观察，病毒粒子直径为27 nm。病毒蛋白似乎可分为四种多肽，分子量分别为81k、70k、66k和62k道尔顿。交叉血清中和试验表明，该病毒在抗原性上与猪细小病毒以及牛和犬细小病毒不同。这些发现以及中和抗体分布调查间接表明，在日本猪群中广泛存在一种在抗原性上可与已知猪细小病毒区分的另一种细小病毒。
