• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

内源性AJAP1与细胞骨架相关，并减弱内皮细胞中的血管生成。

Endogenous AJAP1 associates with the cytoskeleton and attenuates angiogenesis in endothelial cells.

作者信息

Hötte Katharina, Smyrek Isabell, Starzinski-Powitz Anna, Stelzer Ernst H K

机构信息

Physical Biology/Physikalische Biologie (IZN, FB 15), Buchmann Institute for Molecular Life Sciences (BMLS), Cluster of Excellence Frankfurt - Macromolecular Complexes (CEF - MC), Goethe Universität - Frankfurt am Main (Campus Riedberg), Max-von-Laue-Straße 15, Frankfurt am Main D-60438, Germany.

Institute of Cell Biology and Neuroscience, Department of Molecular Cell Biology and Human Genetics, Goethe Universität - Frankfurt am Main (Campus Riedberg), Max-von-Laue-Straße 13, Frankfurt am Main D-60438, Germany.

出版信息

Biol Open. 2017 Jun 15;6(6):723-731. doi: 10.1242/bio.022335.

DOI:10.1242/bio.022335
PMID:28483980
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5483013/
Abstract

The adherens junction associated protein 1 (AJAP1, aka shrew-1) is presumably a type-I transmembrane protein localizing and interacting with the E-cadherin-catenin complex. In various tumors, AJAP1 expression is reduced or lost, including hepatocellular and esophageal squamous cell carcinoma, and glial-derived tumors. The aberrant expression of AJAP1 is associated with alterations in cell migration, invasion, increased tumor growth, and tumor vascularization, suggesting AJAP1 as a putative tumor suppressor. We show that AJAP1 attenuates sprouting angiogenesis by reducing endothelial migration and invasion capacities. Further, we show for the first time that endogenous AJAP1 is associated with the microtubule cytoskeleton. This linkage is independent from cell confluency and stable during angiogenic sprouting Our work suggests that AJAP1 is a putative negative regulator of angiogenesis, reducing cell migration and invasion by interfering with the microtubule network. Based on our results and those of other authors, we suggest AJAP1 as a novel tumor suppressor and diagnostic marker.

摘要

黏附连接相关蛋白1（AJAP1，又名shrew-1）可能是一种I型跨膜蛋白，定位于E-钙黏蛋白-连环蛋白复合体并与其相互作用。在各种肿瘤中，AJAP1的表达降低或缺失，包括肝细胞癌、食管鳞状细胞癌和神经胶质来源的肿瘤。AJAP1的异常表达与细胞迁移、侵袭的改变、肿瘤生长增加以及肿瘤血管生成有关，提示AJAP1是一种假定的肿瘤抑制因子。我们发现AJAP1通过降低内皮细胞的迁移和侵袭能力来减弱芽生血管生成。此外，我们首次表明内源性AJAP1与微管细胞骨架相关。这种联系独立于细胞汇合状态，并且在血管生成芽生过程中保持稳定。我们的研究表明，AJAP1是一种假定的血管生成负调节因子，通过干扰微管网络来减少细胞迁移和侵袭。基于我们的研究结果以及其他作者的结果，我们认为AJAP1是一种新型的肿瘤抑制因子和诊断标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa02/5483013/756b451d9ef3/biolopen-6-022335-g5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa02/5483013/ccb71b0eb50e/biolopen-6-022335-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa02/5483013/96debb0b030b/biolopen-6-022335-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa02/5483013/bf34f692ddf6/biolopen-6-022335-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa02/5483013/be27ba7e318b/biolopen-6-022335-g4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa02/5483013/756b451d9ef3/biolopen-6-022335-g5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa02/5483013/ccb71b0eb50e/biolopen-6-022335-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa02/5483013/96debb0b030b/biolopen-6-022335-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa02/5483013/bf34f692ddf6/biolopen-6-022335-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa02/5483013/be27ba7e318b/biolopen-6-022335-g4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa02/5483013/756b451d9ef3/biolopen-6-022335-g5.jpg

相似文献

1
Endogenous AJAP1 associates with the cytoskeleton and attenuates angiogenesis in endothelial cells.内源性AJAP1与细胞骨架相关，并减弱内皮细胞中的血管生成。
Biol Open. 2017 Jun 15;6(6):723-731. doi: 10.1242/bio.022335.
2
The adherens junction-associated protein 1 is a negative transcriptional regulator of MAGEA2, which potentiates temozolomide-induced apoptosis in GBM.黏着斑连接相关蛋白 1 是 MAGEA2 的负转录调控因子，可增强 GBM 中替莫唑胺诱导的细胞凋亡。
Int J Oncol. 2014 Apr;44(4):1243-51. doi: 10.3892/ijo.2014.2277. Epub 2014 Jan 24.
3
Adherens junctional associated protein-1: a novel 1p36 tumor suppressor candidate in gliomas (Review).黏附连接相关蛋白-1：胶质瘤中一种新的1p36肿瘤抑制候选基因（综述）
Int J Oncol. 2014 Jul;45(1):13-7. doi: 10.3892/ijo.2014.2425. Epub 2014 May 8.
4
Reduced Expression of Adherens Junctions Associated Protein 1 Predicts Recurrence of Hepatocellular Carcinoma After Curative Hepatectomy.黏着连接相关蛋白1表达降低预示根治性肝切除术后肝细胞癌复发
Ann Surg Oncol. 2015 Dec;22 Suppl 3:S1499-507. doi: 10.1245/s10434-015-4695-9. Epub 2015 Jun 30.
5
Expression Patterns of Ezrin and AJAP1 and Clinical Significance in Breast Cancer.埃兹蛋白和AJAP1在乳腺癌中的表达模式及其临床意义
Front Oncol. 2022 Mar 4;12:831507. doi: 10.3389/fonc.2022.831507. eCollection 2022.
6
Downregulation of TET1 Promotes Bladder Cancer Cell Proliferation and Invasion by Reducing DNA Hydroxymethylation of AJAP1.TET1的下调通过降低AJAP1的DNA羟甲基化促进膀胱癌细胞增殖和侵袭。
Front Oncol. 2020 May 21;10:667. doi: 10.3389/fonc.2020.00667. eCollection 2020.
7
AJAP1 affects behavioral changes and GABAR1 level in epileptic mice.AJAP1 影响癫痫小鼠的行为改变和 GABA R1 水平。
Biochem Biophys Res Commun. 2020 Apr 16;524(4):1057-1063. doi: 10.1016/j.bbrc.2020.02.036. Epub 2020 Feb 15.
8
Alternative exon usage creates novel transcript variants of tumor suppressor SHREW-1 gene with differential tissue expression profile.可变外显子的使用产生了具有不同组织表达谱的肿瘤抑制因子SHREW-1基因的新型转录变体。
Biol Open. 2016 Nov 15;5(11):1607-1619. doi: 10.1242/bio.019463.
9
Vascular endothelial growth factor-C promotes vasculogenesis, angiogenesis, and collagen constriction in three-dimensional collagen gels.血管内皮生长因子-C在三维胶原凝胶中促进血管发生、血管生成和胶原收缩。
J Vasc Surg. 2005 Apr;41(4):699-707. doi: 10.1016/j.jvs.2005.01.015.
10
The RASSF8 candidate tumor suppressor inhibits cell growth and regulates the Wnt and NF-kappaB signaling pathways.RASSF8 候选肿瘤抑制因子抑制细胞生长并调节 Wnt 和 NF-κB 信号通路。
Oncogene. 2010 Jul 29;29(30):4307-16. doi: 10.1038/onc.2010.192. Epub 2010 May 31.

引用本文的文献

1
Silencing of expression by promoter methylation activates the Wnt/β-catenin signaling pathway to promote tumor proliferation and metastasis in salivary adenoid cystic carcinoma.启动子甲基化导致的表达沉默激活Wnt/β-连环蛋白信号通路，促进涎腺腺样囊性癌的肿瘤增殖和转移。
Gland Surg. 2023 Jun 30;12(6):834-852. doi: 10.21037/gs-23-127. Epub 2023 Jun 27.
2
Role of Cell-Cell Junctions in Oesophageal Squamous Cell Carcinoma.细胞-细胞连接在食管鳞状细胞癌中的作用。
Biomolecules. 2022 Sep 26;12(10):1378. doi: 10.3390/biom12101378.
3
Expression Patterns of Ezrin and AJAP1 and Clinical Significance in Breast Cancer.

本文引用的文献

1
Alternative exon usage creates novel transcript variants of tumor suppressor SHREW-1 gene with differential tissue expression profile.可变外显子的使用产生了具有不同组织表达谱的肿瘤抑制因子SHREW-1基因的新型转录变体。
Biol Open. 2016 Nov 15;5(11):1607-1619. doi: 10.1242/bio.019463.
2
Adherens junctions associated protein 1 serves as a predictor of recurrence of squamous cell carcinoma of the esophagus.黏着连接相关蛋白 1 可作为食管鳞癌复发的预测指标。
Int J Oncol. 2015 Nov;47(5):1811-8. doi: 10.3892/ijo.2015.3167. Epub 2015 Sep 16.
3
Reduced Expression of Adherens Junctions Associated Protein 1 Predicts Recurrence of Hepatocellular Carcinoma After Curative Hepatectomy.
埃兹蛋白和AJAP1在乳腺癌中的表达模式及其临床意义
Front Oncol. 2022 Mar 4;12:831507. doi: 10.3389/fonc.2022.831507. eCollection 2022.
4
Phenotypic Consequences of Fusions beyond Drug Resistance in High-Grade Serous Ovarian Cancer.高级别浆液性卵巢癌中融合现象超出耐药性的表型后果
Cancers (Basel). 2021 Nov 11;13(22):5644. doi: 10.3390/cancers13225644.
5
Genetic analysis of the molecular regulation of electric fields-guided glia migration.电场导向神经胶质细胞迁移的分子调控的遗传分析。
Sci Rep. 2020 Oct 8;10(1):16821. doi: 10.1038/s41598-020-74085-x.
6
Genome-wide association study and a post replication analysis revealed a promising genomic region and candidate genes for chicken eggshell blueness.全基因组关联研究和后续复制分析揭示了一个有希望的基因组区域和候选基因，用于研究鸡蛋壳蓝色。
PLoS One. 2019 Jan 23;14(1):e0209181. doi: 10.1371/journal.pone.0209181. eCollection 2019.
7
MiR-552 promotes the proliferation, migration and EMT of hepatocellular carcinoma cells by inhibiting AJAP1 expression.miR-552 通过抑制 AJAP1 表达促进肝癌细胞的增殖、迁移和 EMT。
J Cell Mol Med. 2019 Feb;23(2):1541-1552. doi: 10.1111/jcmm.14062. Epub 2018 Dec 30.
黏着连接相关蛋白1表达降低预示根治性肝切除术后肝细胞癌复发
Ann Surg Oncol. 2015 Dec;22 Suppl 3:S1499-507. doi: 10.1245/s10434-015-4695-9. Epub 2015 Jun 30.
4
AJAP1 is dysregulated at an early stage of gliomagenesis and suppresses invasion through cytoskeleton reorganization.AJAP1 在神经胶质瘤发生的早期就失调了，并通过细胞骨架重排来抑制侵袭。
CNS Neurosci Ther. 2014 May;20(5):429-37. doi: 10.1111/cns.12232. Epub 2014 Feb 1.
5
The adherens junction-associated protein 1 is a negative transcriptional regulator of MAGEA2, which potentiates temozolomide-induced apoptosis in GBM.黏着斑连接相关蛋白 1 是 MAGEA2 的负转录调控因子，可增强 GBM 中替莫唑胺诱导的细胞凋亡。
Int J Oncol. 2014 Apr;44(4):1243-51. doi: 10.3892/ijo.2014.2277. Epub 2014 Jan 24.
6
Methylomics analysis identifies epigenetically silenced genes and implies an activation of β-catenin signaling in cervical cancer.甲基化组学分析鉴定出了表观遗传沉默的基因，并提示宫颈癌中β-连环蛋白信号的激活。
Int J Cancer. 2014 Jul 1;135(1):117-27. doi: 10.1002/ijc.28658. Epub 2013 Dec 17.
7
Microtubules coordinate VEGFR2 signaling and sorting.微管协调 VEGFR2 信号转导和分拣。
PLoS One. 2013 Sep 20;8(9):e75833. doi: 10.1371/journal.pone.0075833. eCollection 2013.
8
Deletion or epigenetic silencing of AJAP1 on 1p36 in glioblastoma.胶质母细胞瘤中 1p36 上 AJAP1 的缺失或表观遗传沉默。
Mol Cancer Res. 2012 Feb;10(2):208-17. doi: 10.1158/1541-7786.MCR-10-0109. Epub 2012 Jan 12.
9
Tumor-associated methylation of the putative tumor suppressor AJAP1 gene and association between decreased AJAP1 expression and shorter survival in patients with glioma.假定的肿瘤抑制基因AJAP1的肿瘤相关甲基化以及AJAP1表达降低与胶质瘤患者较短生存期之间的关联。
Chin J Cancer. 2011 Apr;30(4):247-53. doi: 10.5732/cjc.011.10025.
10
The mammary gland vasculature revisited.乳腺血管再探。
J Mammary Gland Biol Neoplasia. 2010 Sep;15(3):319-28. doi: 10.1007/s10911-010-9186-9. Epub 2010 Aug 14.