患颌部肿块病的东部灰袋鼠（大赤袋鼠）的血浆内毒素活性
Plasma endotoxin activity in Eastern grey kangaroos (Macropus giganteus) with lumpy jaw disease.
作者信息
Sotohira Yukari, Suzuki Kazuyuki, Otsuka Marina, Tsuchiya Masakazu, Shimamori Toshio, Nishi Yasunobu, Tsukano Kenji, Asakawa Mitsuhiko
机构信息
School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu, Hokkaido 069-8501, Japan.
Itozu no mori Zoological Park, 4-1-8 Kamiitozu, Kokurakita, Kitakyushu, Fukuoka 803-0845, Japan.
出版信息
J Vet Med Sci. 2017 Jun 29;79(6):1138-1141. doi: 10.1292/jvms.16-0631. Epub 2017 May 8.
Progressive pyogranulomatous osteomyelitis involving the mandible or maxilla of captive macropods, referred to as "Lumpy jaw disease (LJD)", is one of the most significant causes of illness and death in captive macropods. The aim of the present study was to evaluate the relationship between the severity of LJD and plasma endotoxin activity in kangaroos. Plasma samples obtained from moderate (n=24) and severe LJD (n=12), and healthy kangaroos (n=46), were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. Plasma endotoxin activity was measured using the Limulus amebocyte lysate (LAL)-kinetic turbidimetric (KT) assay. Plasma endotoxin activity was higher in kangaroos with severe LJD (0.199 ± 0.157 EU/ml) than in those with moderate LJD (0.051 ± 0.012 EU/ml, P<0.001) and healthy controls (0.057 ± 0.028 EU/ml, P<0.001). Our results suggest that the severity of LJD in captive macropods may be related to the plasma endotoxin activity.
侵袭圈养有袋目动物下颌骨或上颌骨的进行性脓性肉芽肿性骨髓炎，被称为“肿块颌病（LJD）”，是圈养有袋目动物发病和死亡的最重要原因之一。本研究的目的是评估袋鼠LJD严重程度与血浆内毒素活性之间的关系。从患有中度LJD（n = 24）和重度LJD（n = 12）的袋鼠以及健康袋鼠（n = 46）采集的血浆样本，用无内毒素水按1:20稀释，并加热至80°C 10分钟。使用鲎试剂（LAL）动力学比浊法（KT）测定血浆内毒素活性。重度LJD袋鼠的血浆内毒素活性（0.199±0.157 EU/ml）高于中度LJD袋鼠（0.051±0.012 EU/ml，P<0.001）和健康对照袋鼠（0.057±0.028 EU/ml，P<0.001）。我们的结果表明，圈养有袋目动物LJD的严重程度可能与血浆内毒素活性有关。
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