Yu Ruoxi, Yang Yin, Han Yuanyuan, Hou Pengwei, Li Yingshuai, Li Siqi, Wang Qi
Health Preservation Institute, Beijing University of Chinese Medicine, Beijing, China.
Center for Studies in Constitution Research of Traditional Chinese Medicine, School of Preclinical Medicine, Beijing, China.
Evid Based Complement Alternat Med. 2016;2016:1493098. doi: 10.1155/2016/1493098. Epub 2016 Nov 15.
. Differences among healthy subjects and associated disease risks are of substantial interest in clinical medicine. According to the theory of "constitution-disease correlation" in traditional Chinese medicine, we try to find out if there is any connection between intolerance of cold in Yang deficiency constitution and molecular evidence and if there is any gene expression basis in specific disorders. . Peripheral blood mononuclear cells were collected from Chinese Han individuals with Yang deficiency constitution ( = 20) and balanced constitution ( = 8) (aged 18-28) and global gene expression profiles were determined between them using the Affymetrix HG-U133 Plus 2.0 array. . The results showed that when the fold change was ≥1.2 and ≤ 0.05, 909 genes were upregulated in the Yang deficiency constitution, while 1189 genes were downregulated. According to our research differential genes found in Yang deficiency constitution were usually related to lower immunity, metabolic disorders, and cancer tendency. . Gene expression disturbance exists in Yang deficiency constitution, which corresponds to the concept of constitution and gene classification. It also suggests people with Yang deficiency constitution are susceptible to autoimmune diseases, enteritis, arthritis, metabolism disorders, and cancer, which provides molecular evidence for the theory of "constitution-disease correlation."
健康受试者之间的差异及相关疾病风险在临床医学中具有重大意义。根据中医“体质 - 疾病相关性”理论，我们试图探究阳虚体质的畏寒现象与分子证据之间是否存在关联，以及特定病症中是否存在基因表达基础。从18 - 28岁的中国汉族阳虚体质个体（n = 20）和平衡体质个体（n = 8）中采集外周血单个核细胞，并使用Affymetrix HG - U133 Plus 2.0芯片测定两者之间的全基因组表达谱。结果显示，当倍数变化≥1.2且P≤0.05时，阳虚体质中有909个基因上调，1189个基因下调。根据我们的研究，在阳虚体质中发现的差异基因通常与免疫力低下、代谢紊乱和癌症倾向有关。阳虚体质存在基因表达紊乱，这与体质和基因分类的概念相符。这也表明阳虚体质者易患自身免疫性疾病、肠炎、关节炎、代谢紊乱和癌症，为“体质 - 疾病相关性”理论提供了分子证据。
