点状气单胞菌噬菌体的分离与鉴定
Isolation and Characterization of an Aeromonas punctata Bacteriophage.
作者信息
Kai Cheng, Denglan Zhang, Jingxuan Deng, Yijun Zhao
机构信息
Key Laboratory of Ecological Remedlation of Lakes and Rivers and Algal Utilization of Hubei Province, College of Resources and Environmental Engineering, Hubei University of Technology, Wuhan, Hubei, 430068, P.R. China.
College of Life Science, Central China Normal University, Wuhan, Hubei, 430079, P.R. China.
出版信息
Open Biomed Eng J. 2015 Aug 31;9:185-187. doi: 10.2174/1874120701509010185. eCollection 2015.
An bacteriophage, named as DH1, was isolated from East Lake, Wuhan city, China. Morphologically, phage DH1 showed a typical structure consisting of an isometric head (50 nm in diameter) and a visible tail. The bacteriophage had a latent period of about 90 minutes and an average burst size of about 125 PFU•Cell. Restriction enzyme pattern of the bacteriophage's genome showed that the genome is a double-stranded DNA and about 34kb in size. The sequenced genomic fragments showed highly similarities to gp04 and gp16 sequence of other bacteriophages at protein level.
从中国武汉市东湖分离出一种名为DH1的噬菌体。形态学上，噬菌体DH1呈现出典型的结构，由一个等轴头（直径50纳米）和一条可见的尾部组成。该噬菌体的潜伏期约为90分钟，平均裂解量约为125噬菌斑形成单位·细胞。噬菌体基因组的限制性酶切图谱表明，基因组是双链DNA，大小约为34kb。测序的基因组片段在蛋白质水平上与其他噬菌体的gp04和gp16序列高度相似。
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