Nazerian K, Silva R F
U.S. Department of Agriculture, Regional Poultry Research Laboratory, East Lansing, Michigan 48823.
Avian Dis. 1988 Jul-Sep;32(3):486-93.
The MDTC-RP30 lymphoblastoid cell line established from Marek's disease (MD) tumors in turkeys consisted of a heterogeneous population of cells 10 to 25 micron in diameter. Large-cell fractions obtained from a bovine fetal serum gradient had a higher titer of cell-associated MD virus (MDV) than the small-cell fractions. Seven single-cell clones were established from MDTC-RP30 cell line: two consisted of large cells, and the other clones consisted of small cells. Infectious MDV was rescued from large-cell clones in chicken embryo fibroblast cultures but not from small-cell clones. All clones contained MDV DNA sequences when hybridized against cloned MDV DNA. All clones were positive for a Marek's-disease-tumor-associated surface antigen and surface immunoglobulins. All but two small-cell clones caused MD in susceptible chickens. The two large-cell-type clones were uniformly tetraploid, whereas one small-cell clone was diploid and the four others were a mixture of diploid and tetraploid, with an occasional triploid cell. Evidence of translocation involving the male (Z) chromosome and the chromosome #3 was seen in one clone. These results suggest that MDV transforms different subpopulations of lymphocytes.
从患马立克氏病（MD）的火鸡肿瘤中建立的MDTC-RP30淋巴母细胞系由直径为10至25微米的异质细胞群体组成。从牛胎血清梯度中获得的大细胞部分比小细胞部分具有更高滴度的细胞相关MD病毒（MDV）。从MDTC-RP30细胞系中建立了7个单细胞克隆：2个由大细胞组成，其他克隆由小细胞组成。在鸡胚成纤维细胞培养物中从大细胞克隆中拯救出感染性MDV，但从小细胞克隆中未拯救出。当与克隆的MDV DNA杂交时，所有克隆都含有MDV DNA序列。所有克隆对马立克氏病肿瘤相关表面抗原和表面免疫球蛋白均呈阳性。除两个小细胞克隆外，所有克隆均在易感鸡中引起MD。两个大细胞型克隆均为四倍体，而一个小细胞克隆为二倍体，其他四个为二倍体和四倍体的混合物，偶尔有三倍体细胞。在一个克隆中观察到涉及雄性（Z）染色体和3号染色体易位的证据。这些结果表明MDV转化淋巴细胞的不同亚群。
