Kitamoto N, Ikuta K, Kato S, Hirai K
Biken J. 1980 Mar;23(1):1-8.
A cell line tentatively designated as MDCC-BO1(T), was established from spleen cells of an apparently healthy chicken inoculated with herpesvirus of turkey (HVT). BO1(T) cells were T lymphoblastoid cells and the more than 95% of them had Marek's disease (MD) tumor-associated surface antigen (MATSA). However, no viral internal antigens or membrane antigens could be demonstrated in them by immunofluorescence tests using chicken anti-HVT and -MD virus (MDV) sera. The virus could be rescued from BO1(T) cells by co-cultivation with chick embryo fibroblasts (CEF). The DNA of the rescued virus was characterized as HVT DNA by its sedimentation profile in a neutral glycerol gradient and its endonuclease Hind III cleavage-pattern. Ultrastructural studies on CEF infected with the rescued virus revealed the presence of HVT-like virions. However, DNA-DNA reassociation kinetics showed that the BO1(T) cells contained a few copies of NVT and also MDV genomes.
一株暂命名为MDCC - BO1(T)的细胞系，是从接种火鸡疱疹病毒（HVT）的一只外观健康鸡的脾脏细胞中建立的。BO1(T)细胞是T淋巴母细胞样细胞，其中超过95%的细胞具有马立克氏病（MD）肿瘤相关表面抗原（MATSA）。然而，使用鸡抗HVT和 - MD病毒（MDV）血清进行免疫荧光试验，在这些细胞中未检测到病毒内部抗原或膜抗原。通过与鸡胚成纤维细胞（CEF）共培养，可从BO1(T)细胞中拯救出病毒。通过其在中性甘油梯度中的沉降图谱及其核酸内切酶Hind III切割模式，将拯救出的病毒DNA鉴定为HVT DNA。对感染拯救出病毒的CEF进行超微结构研究，发现存在HVT样病毒粒子。然而，DNA - DNA重缔合动力学表明，BO1(T)细胞含有少量NVT以及MDV基因组的拷贝。
