Universidad Autonoma de Nuevo Leon, Facultad de Ciencias Biologicas, San Nicolas de los Garza, Mexico.
Universidad de los Andes, Nucleo Universitario Rafael Rangel, Pampanito, Trujillo, Venezuela.
Pest Manag Sci. 2017 Nov;73(11):2287-2293. doi: 10.1002/ps.4609. Epub 2017 Jul 24.
Resistance to insecticides through one or several mechanisms has a cost for an insect in various parameters of its biological cycle. The present study evaluated the effect of deltamethrin on detoxifying enzymes and biological parameters in a population of Aedes aegypti selected for 15 generations. The enzyme activities of alpha- and beta-esterases, mixed-function oxidases and glutathione-S-transferases were determined during selection, along with biological parameters.
Overexpression of mixed-function oxidases as a mechanism of metabolic resistance to deltamethrin was found. There were decreases in percentages of eggs hatching, pupation and age-specific survival and in total survival at the end of the selection (F ). Although age-specific fecundity was not affected by selection with deltamethrin, total fertility, together with lower survival, significantly affected gross reproduction rate, gradually decreasing due to deltamethrin selection. Similarly, net reproductive rate and intrinsic growth rate were affected by selection.
Alterations in life parameters could be due to the accumulation of noxious effects or deleterious genes related to detoxifying enzymes, specifically those coding for mixed-function oxidases, along with the presence of recessive alleles of the V1016I and F1534C mutations, associating deltamethrin resistance with fitness cost in Ae. aegypti. © 2017 Society of Chemical Industry.
通过一种或多种机制对杀虫剂产生抗药性会使昆虫在其生物周期的各种参数上付出代价。本研究评估了 15 代选择的埃及伊蚊种群中拟除虫菊酯对解毒酶和生物参数的影响。在选择过程中，测定了α-和β-酯酶、多功能氧化酶和谷胱甘肽-S-转移酶的酶活性，以及生物参数。
发现了一种对拟除虫菊酯代谢抗性的多功氧化酶过表达机制。在选择结束时，孵化率、蛹化率和特定年龄存活率以及总存活率的百分比下降（F）。虽然拟除虫菊酯选择对特定年龄的生育力没有影响，但总生育率以及较低的存活率显著影响总繁殖率，由于拟除虫菊酯的选择，其逐渐降低。同样，净生殖率和内在增长率也受到选择的影响。
生命参数的改变可能是由于与解毒酶相关的有害或有害基因的积累，特别是那些编码多功能氧化酶的基因，以及 V1016I 和 F1534C 突变的隐性等位基因的存在，将拟除虫菊酯抗性与埃及伊蚊的适应成本联系起来。© 2017 化学工业协会。
