委内瑞拉埃及伊蚊电压门控钠通道基因中V1016I和F1534C突变的频率
Frequency of V1016I and F1534C mutations in the voltage-gated sodium channel gene in Aedes aegypti in Venezuela.
作者信息
Alvarez Leslie C, Ponce Gustavo, Saavedra-Rodriguez Karla, Lopez Beatriz, Flores Adriana E
机构信息
Universidad Autonoma de Nuevo Leon, Facultad de Ciencias Biologicas, San Nicolas de los Garza, Mexico; Universidad de los Andes, Nucleo Universitario Rafael Rangel. Villa Universitaria Pampanito, estado Trujillo, 3102, Venezuela.
出版信息
Pest Manag Sci. 2015 Jun;71(6):863-9. doi: 10.1002/ps.3846. Epub 2014 Jul 15.
BACKGROUND
The V1016I and F1534C mutations in the voltage-gated sodium channel gene have been associated with resistance to pyrethroids and DDT in Aedes aegypti mosquitoes. A study was carried out to determine the frequency of I1016 and C1534 by real-time PCR in five natural populations of Ae. aegypti in Venezuela during 2008, 2010 and 2012, as well as in a strain selected with 0.14 µg of deltamethrin for 15 generations.
RESULTS
In natural populations, frequencies of I1016 varied between 0.01 and 0.37, and frequencies of C1534 between 0.35 and 1.0. In the Pampanito strain, the frequency of I1016 increased from 0.02 in F1 up to 0.5 in F15 and from 0.35 up to fixation for C1534 after selection with deltamethrin.
CONCLUSION
The results showed that C1534 frequencies are higher than I1016 frequencies in natural populations of Ae. aegypti in Venezuela, and that deltamethrin selected the C1534 more rapidly than I1016.
背景
电压门控钠通道基因中的V1016I和F1534C突变与埃及伊蚊对拟除虫菊酯和滴滴涕的抗性有关。开展了一项研究，通过实时PCR测定2008年、2010年和2012年委内瑞拉五个埃及伊蚊自然种群以及用0.14微克溴氰菊酯选择15代的品系中I1016和C1534的频率。
结果
在自然种群中，I1016的频率在0.01至0.37之间变化，C1534的频率在0.35至1.0之间变化。在潘帕尼托品系中，用溴氰菊酯选择后，I1016的频率从F1代的0.02增加到F15代的0.5，C1534从0.35增加到固定值。
结论
结果表明，在委内瑞拉埃及伊蚊的自然种群中，C1534的频率高于I1016的频率，并且溴氰菊酯对C1534的选择比对I1016的选择更快。
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