Yiannakou Ch, Simitzi Ch, Manousaki A, Fotakis C, Ranella A, Stratakis E
Institute of Electronic Structure and Laser, Foundation for Research and Technology-Hellas, Heraklion, 71110, Crete, Greece. Department of Physics, University of Crete, Heraklion, 71003, Crete, Greece.
Biofabrication. 2017 May 31;9(2):025024. doi: 10.1088/1758-5090/aa71c6.
The surface topography of biomaterials can have an important impact on cellular adhesion, growth and proliferation. Apart from the overall roughness, the detailed morphological features, at all length scales, significantly affect the cell-biomaterial interactions in a plethora of applications including structural implants, tissue engineering scaffolds and biosensors. In this study, we present a simple, one-step direct laser patterning technique to fabricate nanoripples and dual-rough hierarchical micro/nano structures to control SW10 cell attachment and migration. It is shown that, depending on the laser processing conditions, distinct cell-philic or cell-repellant patterned areas can be attained with a desired motif. We envisage that our technique could enable spatial patterning of cells in a controllable manner, giving rise to advanced capabilities in cell biology research.
生物材料的表面形貌会对细胞的黏附、生长和增殖产生重要影响。除了整体粗糙度外，所有长度尺度上的详细形态特征在包括结构植入物、组织工程支架和生物传感器在内的众多应用中，都会显著影响细胞与生物材料的相互作用。在本研究中，我们提出了一种简单的一步直接激光图案化技术，用于制造纳米波纹和双粗糙分级微/纳米结构，以控制SW10细胞的附着和迁移。结果表明，根据激光加工条件，可以获得具有所需图案的不同亲细胞或拒细胞图案区域。我们设想，我们的技术能够以可控方式实现细胞的空间图案化，从而在细胞生物学研究中带来先进的能力。
