Durda P J, Leece B, Jenoski A, Rabin H, Fisher A, Wong-Staal F
E.I. duPont de Nemours & Co., Medical Products Department, N. Billerica, MA 01862.
AIDS Res Hum Retroviruses. 1988 Oct;4(5):331-42. doi: 10.1089/aid.1988.4.331.
We have used short synthetic peptides, 12 and 13 amino acids in length, conjugated to carrier proteins to develop monoclonal antibodies (MAb) to the envelope glycoprotein of 120 (kD) (gp120) and the 3' open reading frame protein (3-orf) of the human immunodeficiency virus type 1 (HIV-1). The peptides employed were chosen because of their strong hydrophilicity and in the case of the gp120 peptide because it represents a highly conserved hydrophilic region in the envelope protein. The MAb developed displayed appropriate specificities with their respective peptides and reacted with appropriate HIV-1 components (i.e., a 120 kD glycoprotein and a 27 kD protein, respectively) as determined by Western blot analysis. In indirect immunofluorescence assays the MAb strongly stained syncytia present in cultures of HTLV-3B-infected H9 cells. The MAb to the envelope component reacted with the RF isolate of HIV-1, as well as with the 3B isolate in immunofluorescence.
我们使用了长度为12和13个氨基酸的短合成肽，将其与载体蛋白偶联，以制备针对人类免疫缺陷病毒1型（HIV-1）包膜糖蛋白120（kD）（gp120）和3'开放阅读框蛋白（3-orf）的单克隆抗体（MAb）。所使用的肽因其强亲水性而被选用，就gp120肽而言，是因为它代表包膜蛋白中一个高度保守的亲水区域。所制备的单克隆抗体对各自的肽表现出适当的特异性，并通过蛋白质印迹分析确定，它们分别与适当的HIV-1组分（即120 kD糖蛋白和27 kD蛋白）发生反应。在间接免疫荧光试验中，这些单克隆抗体强烈染色HTLV-3B感染的H9细胞培养物中出现的多核巨细胞。针对包膜成分的单克隆抗体在免疫荧光中与HIV-1的RF分离株以及3B分离株发生反应。
