Laboratoire d'Ecologie Microbienne, FSNV, Université de Bejaia, 06000 Bejaia, Algeria; Unité de Recherche sur les Maladies Infectieuses et Tropicales émergentes (URMITE), UM 63, CNRS 7278, IRD 198, INSERM 1095, IHU Méditerranée Infection, Faculté de Médecine et de Pharmacie, Aix-Marseille-Université, Marseille, France.
Laboratoire d'Ecologie Microbienne, FSNV, Université de Bejaia, 06000 Bejaia, Algeria.
Water Res. 2017 Sep 1;120:185-189. doi: 10.1016/j.watres.2017.04.073. Epub 2017 Apr 30.
The spread of carbapenemase-producing Enterobacteriaceae (CPE) is a significant problem for healthcare worldwide. The prevalence of carbapenem-resistant Enterobacteriaceae (CPE) in water environments in Algeria are unknown. The aim of this study was to screen for the presence of CPE isolates in the Soummam River in Bejaia, Algeria. Isolates of Enterobacteriaceae recovered from twelve samples of river water and showing reduced susceptibility to carbapenems were included in this study. The isolates were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Isolates were subjected to antimicrobial susceptibility testing and the modified Carba NP test. Carbapenemase and extended-spectrum β-lactamase (ESBL) determinants were studied by PCR amplification and sequencing. The clonal relatedness between isolates was studied by Multilocus Sequence Typing (MLST) method. A total of 20 carbapenem-resistant Enterobacteriaceae strains were included in this study, identified as Escherichia coli (n = 12), Klebsiella pneumoniae (n = 3), Raoultella ornithinolytica (n = 3), Citrobacter freundii (n = 1) and Citrobacter braakii (n = 1). Carbapenemase genes identified in this study included bla, observed in 17 isolates (9 E. coli, 3 K. pneumoniae, 3 R. ornithinolytica, 1 C. freundii and 1 C. braakii), and bla, a variant of bla, was found in three E. coli isolates. MLST showed that 12 E. coli strains belonged to six different sequence types (ST559, ST38, ST212, ST3541, 1972 and ST2142), and we identified three different STs in K. pneumoniae isolates, including ST133, ST2055, and a new sequence type: ST2192. This study showed the presence of OXA-48-like-producing Enterobacteriaceae in water environments and highlighted the potential role of aquatic environments as reservoirs of clinically relevant antimicrobial-resistant bacteria, with the potential to spread throughout the community.
产碳青霉烯酶肠杆菌科（CPE）的传播是全球卫生保健面临的重大问题。阿尔及利亚水环境中耐碳青霉烯肠杆菌科（CPE）的流行情况尚不清楚。本研究的目的是在阿尔及利亚贝贾亚的 Soummam 河检测 CPE 分离株的存在情况。从 12 个河水样本中回收的对碳青霉烯类药物敏感性降低的肠杆菌科分离株包括在本研究中。通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）对分离株进行鉴定。对分离株进行了抗菌药物敏感性试验和改良的 Carba NP 试验。通过 PCR 扩增和测序研究了碳青霉烯酶和超广谱β-内酰胺酶（ESBL）决定簇。通过多位点序列分型（MLST）方法研究了分离株之间的克隆相关性。本研究共纳入 20 株耐碳青霉烯肠杆菌科菌株，鉴定为大肠埃希菌（n=12）、肺炎克雷伯菌（n=3）、产酸克雷伯菌（n=3）、弗氏柠檬酸杆菌（n=1）和布氏柠檬酸杆菌（n=1）。本研究中发现的碳青霉烯酶基因包括 bla，在 17 株分离株中观察到（9 株大肠埃希菌、3 株肺炎克雷伯菌、3 株产酸克雷伯菌、1 株弗氏柠檬酸杆菌和 1 株布氏柠檬酸杆菌），bla，是 bla 的变体，在 3 株大肠埃希菌分离株中发现。MLST 显示 12 株大肠埃希菌菌株属于六个不同的序列类型（ST559、ST38、ST212、ST3541、1972 和 ST2142），我们在肺炎克雷伯菌分离株中发现了三个不同的 ST，包括 ST133、ST2055 和一个新的序列类型：ST2192。本研究表明，水生环境中存在产 OXA-48 样碳青霉烯酶肠杆菌科，并且强调了水生环境作为临床相关抗微生物耐药菌储库的潜在作用，这些细菌有可能在整个社区传播。
