Fuga Bruna, Neves Ingrith, Fontana Herrison, Bispo Jessica, Sano Elder, Cardenas-Arias Adriana, Esposito Fernanda, Cardoso Brenda, Ienne Susan, Sellera Fábio P, Lincopan Nilton
Department of Clinical Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil.
One Health Brazilian Resistance Project (OneBr), São Paulo, Brazil.
Front Microbiol. 2025 Jul 8;16:1593887. doi: 10.3389/fmicb.2025.1593887. eCollection 2025.
Contamination of food by antimicrobial-resistant pathogens poses significant risk to consumers and environment, potentially leading to foodborne illnesses, silent colonization, and dissemination of antibiotic-resistant bacteria across geographic borders.
This study analyzed 32 retail meat samples (12 chicken, 10 beef, and 10 pork) from conventional (CN) and antibiotic-free (AF) production systems in Brazil, assessing WHO bacterial priority pathogens through whole genome sequencing and microbiological methods.
Five broad-spectrum cephalosporin-resistant strains were isolated from AF chicken meat, including four extended-spectrum -lactamase (ESβL) producers belonging to sequence types (STs) ST117, ST443, ST1559 and ST3258, and one pAmpC producer carrying the gene and belonging to ST57. On the other hand, four strains resistant to 3rd generation cephalosporins were identified in CN chicken meat, being three ESβL producers of ST38, ST2179 and ST2040, and one pAmpC producer belonging to ST350. Genes conferring resistance to hazardous heavy metals, disinfectants, and pesticides were identified, whereas virulent potential of ST350 and ST2040 was predicted. Noteworthy, ST38 was genomically related to lineages previously identified in poultry (North America) and polluted environments (Europe), supporting an intercontinental dissemination within a One Health framework.
Our findings reinforce the need for continuous surveillance of WHO critical priority pathogens in the chicken meat supply chain from different production systems.
抗菌药物耐药性病原体污染食品对消费者和环境构成重大风险，可能导致食源性疾病、无症状定植以及耐药细菌跨越地理边界传播。
本研究分析了巴西传统（CN）和无抗生素（AF）生产系统的32份零售肉类样本（12份鸡肉、10份牛肉和10份猪肉），通过全基因组测序和微生物学方法评估世界卫生组织细菌重点病原体。
从AF鸡肉中分离出5株对广谱头孢菌素耐药的菌株，包括4株属于序列类型（STs）ST117、ST443、ST1559和ST3258的超广谱β-内酰胺酶（ESβL）产生菌，以及1株携带基因且属于ST57的pAmpC产生菌。另一方面，在CN鸡肉中鉴定出4株对第三代头孢菌素耐药的菌株，其中3株是ST38、ST2179和ST2040的ESβL产生菌，1株是属于ST350的pAmpC产生菌。鉴定出了赋予对有害重金属、消毒剂和农药耐药性的基因，同时预测了ST350和ST2040的毒力潜力。值得注意的是，ST38在基因组上与先前在家禽（北美）和污染环境（欧洲）中鉴定出的谱系相关，支持在“同一个健康”框架内的洲际传播。
我们的研究结果强化了对来自不同生产系统的鸡肉供应链中世界卫生组织关键重点病原体进行持续监测的必要性。
