Xu Lingyang, He Yanghua, Ding Yi, Sun Guirong, Carrillo Jose Adrian, Li Yaokun, Ghaly Mona M, Ma Li, Zhang Huanmin, Liu George E, Song Jiuzhou
Department of Animal & Avian Sciences, University of Maryland, College Park, MD 20742, USA.
Animal Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA.
Int J Mol Sci. 2017 May 9;18(5):1020. doi: 10.3390/ijms18051020.
Marek's Disease (MD) is a highly contagious pathogenic and oncogenic disease primarily affecting chickens. Chicken Lines 63 and 72, as well as their recombinant congenic strains (RCS) with varied susceptibility to MD, are ideal models to study the complex mechanisms of genetic resistance to MD. In this study, we investigated copy number variation (CNV) in these inbred chicken lines using the Affymetrix Axiom HD 600 K SNP genotyping array. We detected 393 CNV segments across all ten chicken lines, of which 12 CNVs were specifically identified in Line 72. We then assessed genetic structure based on CNV and observed markedly different patterns. Finally, we validated two deletion events in Line 72 and correlated them with genes expression using qPCR and RNA-seq, respectively. Our combined results indicated that these two CNV deletions were likely to contribute to MD susceptibility.
马立克氏病（MD）是一种主要影响鸡的高度传染性致病性和致瘤性疾病。63系和72系鸡及其对MD具有不同易感性的重组近交系（RCS）是研究MD遗传抗性复杂机制的理想模型。在本研究中，我们使用Affymetrix Axiom HD 600 K SNP基因分型芯片研究了这些近交系鸡的拷贝数变异（CNV）。我们在所有十个鸡系中检测到393个CNV片段，其中12个CNV在72系中被特异性鉴定。然后，我们基于CNV评估了遗传结构，并观察到明显不同的模式。最后，我们验证了72系中的两个缺失事件，并分别使用qPCR和RNA-seq将它们与基因表达相关联。我们的综合结果表明，这两个CNV缺失可能导致对MD的易感性。
Zotero 插件
