Li You, Huang Yongquan, Liang Xue, Long Bingshuang, Chen Shiyi, Lian Jiahao, Wei Yi, Zhang Zhiyong, Qin Jian
Department of Occupational and Environmental Health, Faculty of Public Health, Dali University, Dali 671000, China.
Department of Occupational and Environmental Health, School of Public Health, Guangxi Medical University, Shuangyong Road No.22, Nanning 530021, China.
Int J Environ Res Public Health. 2017 May 9;14(5):505. doi: 10.3390/ijerph14050505.
This study aims to determine the association between the apolipoprotein C-I polymorphism and the longevity and genetic variants in ApoC-I that can influence the serum lipid levels in Bama. ApoC-I genotypes were determined by Taqman single nucleotide polymorphism (SNP) genotyping assays in 178 long-lived inhabitants (longevity group aged from 90 to 110 years), 147 healthy controls (Control 1 group aged from 40 to 79 years old) from Bama County, and 190 healthy controls (Control 2 group aged from 40 to 79 years old) from Nandan County without a family history of longevity. Statistical analysis was conducted using SPSS 16.0. All genotype distributions of rs584007 and rs4420638 were consistent with the Hardy-Weinberg equilibrium ( > 0.05). Significant differences were observed in the frequencies of the three genotypes (GG, AG, and AA) among the longevity and the two control groups (χ² = 11.238, = 0.024) for rs584007. No significant differences were observed in the frequencies of the three genotypes (GG, AG, and AA) among the longevity and the two control groups (χ² = 4.587, = 0.318) for rs4420638. The levels of total cholesterol (TC), triglycerides (TG), high-density lipoprotein-cholesterol (HDL-c), and low-density lipoprotein-cholesterol (LDL-c) were not different among the three genotypes of rs584007 in the three groups. The levels of HDL-c for GG, AG, and AA were significantly different (the highest being in the longevity group), while the levels of TG for AA and AG genotypes (the lowest being in the longevity group) and the levels of LDL-c for AG were significantly different ( < 0.05) among the three groups for rs584007. The levels of TG and HDL-c were significantly different among the three rs4420638 genotypes in the longevity group. The levels of TC for GG, AG, and AA were significantly different in the Control 2 group, while the levels of TG and HDL-c for AA and AG genotypes were significantly different ( < 0.05) among the three groups for rs4420638. The level of HDL-c was highest in the longevity group for AA and AG genotypes, and the level of TG was highest in the Control 2 group for rs4420638. Serum lipid parameters were related to environmental factors, including age, gender, BMI, DBP, SBP, rs4420638, and rs584007. The ApoC-I polymorphism might be one of the genetic factors of longevity in Bama. The ApoC-I rs4420638 and rs584007 SNPs are associated with serum TG and HDL-c levels in the longevous population.
本研究旨在确定载脂蛋白C-I多态性与巴马地区长寿及影响血清脂质水平的载脂蛋白C-I基因变异之间的关联。通过Taqman单核苷酸多态性（SNP）基因分型检测法，对178名长寿居民（年龄在90至110岁的长寿组）、来自巴马县的147名健康对照者（年龄在40至79岁的对照1组）以及来自南丹县且无长寿家族史的190名健康对照者（年龄在40至79岁的对照2组）的载脂蛋白C-I基因型进行了测定。使用SPSS 16.0进行统计分析。rs584007和rs4420638的所有基因型分布均符合哈迪-温伯格平衡（>0.05）。对于rs584007，在长寿组和两个对照组的三种基因型（GG、AG和AA）频率上观察到显著差异（χ² = 11.238，P = 0.024）。对于rs4420638，在长寿组和两个对照组的三种基因型（GG、AG和AA）频率上未观察到显著差异（χ² = 4.587，P = 0.318）。三组中rs584007三种基因型的总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-c）和低密度脂蛋白胆固醇（LDL-c）水平无差异。对于rs584007，三组中GG、AG和AA的HDL-c水平存在显著差异（长寿组最高），AA和AG基因型的TG水平（长寿组最低）以及AG的LDL-c水平存在显著差异（P<0.05）。长寿组中rs4420638三种基因型的TG和HDL-c水平存在显著差异。对照2组中GG、AG和AA的TC水平存在显著差异，对于rs4420638，三组中AA和AG基因型的TG和HDL-c水平存在显著差异（P<0.05）。对于rs4420638，长寿组中AA和AG基因型的HDL-c水平最高，对照2组中TG水平最高。血清脂质参数与环境因素相关，包括年龄、性别、BMI、舒张压（DBP）、收缩压（SBP）、rs4420638和rs584007。载脂蛋白C-I多态性可能是巴马地区长寿的遗传因素之一。载脂蛋白C-I的rs4420638和rs584007单核苷酸多态性与长寿人群的血清TG和HDL-c水平相关。
