Li You, Liang Guiyun, Shi Liwei, Liang Xue, Long Bingshuang, Qin Jian, Zhang Zhiyong
Department of Occupational and Environmental Health, School of Public Health, Guangxi Medical University, Nanning, Guangxi, China (mainland).
Med Sci Monit. 2016 Dec 27;22:5154-5162. doi: 10.12659/msm.898231.
BACKGROUND The present study was performed to identify the association of PON1 rs662 polymorphism with serum lipid levels and human longevity in the Bama Zhuang population. MATERIAL AND METHODS PON1 genotypes were determined by Taqman SNP Genotyping Assays in 110 long-lived inhabitants (longevity group, aged 90-110 years), 110 healthy inhabitants in Bama County (control 1 group, aged 43-82 years) and 110 healthy inhabitants in Nandan County (control 2 group, aged 28-82 years) without family history of longevity. RESULTS BMI (body mass index) and TG (serum total triglyceride) level were lower in the longevity group than in the two control groups, while the contents of serum LDL-c (low-density lipoprotein cholesterol) and HDL-c (high-density lipoprotein cholesterol) and the levels of SBP (systolic blood pressure) and DBP (diastolic blood pressure) in the longevity group were higher than in the two control groups (p<0.01). Significant differences in the frequencies of three genotypes (GG, AG, and AA) were observed between the longevity group and control 2 group (χ²=15.190, p=0.001). The minor allele frequency (MAF) of rs662 was significantly higher in the longevity group than in the two control groups. The levels of HDL-c in the longevity group were different among the three genotypes (p<0.05). The levels of TG for GG and GG+AG genotypes were significantly different, while the levels of TC (total cholesterol) and HDL-c for AG and GG+AG genotypes were significantly different among the three groups (p<0.05). Serum lipid parameters were correlated with several environmental factors, including age, gender, DBP, SBP, and BMI. The association of PON1 rs662 polymorphism and serum lipid levels was different among the three groups. CONCLUSIONS PON1 polymorphism might be one of the genetic factors of longevity in the Bama Zhuang population. The PON1 rs662 SNP (single nucleotide polymorphism) was associated with serum HDL-c levels in the longevity group.
本研究旨在确定巴马壮族人群中对氧磷酶1（PON1）基因rs662多态性与血脂水平及人类长寿之间的关联。
采用Taqman SNP基因分型检测法，对110名长寿居民（长寿组，年龄90 - 110岁）、110名巴马县健康居民（对照组1，年龄43 - 82岁）和110名南丹县健康居民（对照组2，年龄28 - 82岁）进行PON1基因分型，这些居民均无长寿家族史。
长寿组的体重指数（BMI）和血清总甘油三酯（TG）水平低于两个对照组，而长寿组的血清低密度脂蛋白胆固醇（LDL - c）和高密度脂蛋白胆固醇（HDL - c）含量以及收缩压（SBP）和舒张压（DBP）水平高于两个对照组（p<0.01）。在长寿组和对照组2之间观察到三种基因型（GG、AG和AA）频率存在显著差异（χ² = 15.190，p = 0.001）。rs662的次要等位基因频率（MAF）在长寿组中显著高于两个对照组。长寿组中三种基因型的HDL - c水平存在差异（p<0.05）。GG和GG + AG基因型的TG水平存在显著差异，而三组之间AG和GG + AG基因型的总胆固醇（TC）和HDL - c水平存在显著差异（p<0.05）。血脂参数与包括年龄、性别、DBP、SBP和BMI在内的多个环境因素相关。三组中PON1 rs662多态性与血脂水平的关联不同。
PON1多态性可能是巴马壮族人群长寿的遗传因素之一。PON1 rs662单核苷酸多态性（SNP）与长寿组的血清HDL - c水平相关。
