大鼠血浆中地高辛样免疫反应性与钠钾ATP酶抑制活性的解离

Dissociation of digoxin-like immunoreactivity and Na+,K+-ATPase inhibitory activity in rat plasma.

作者信息

Yamada K, Goto A, Ishii M, Yoshioka M, Sugimoto T

机构信息

Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.

出版信息

Experientia. 1988 Dec 1;44(11-12):992-3. doi: 10.1007/BF01939897.

DOI:10.1007/BF01939897

PMID:2848712

Abstract

We measured endogenous digitalis-like factor (EDF) in rat plasma during acute saline infusion by two different procedures. Na+,K+-ATPase inhibitory activity in the rat plasma significantly increased during saline loading (7.8 +/- 2.2 vs 2.5 +/- 0.9%, with and without acute saline loading, respectively, p less than 0.05). On the other hand, the plasma digoxin-like immunoreactivity significantly decreased during acute saline loading (16.9 +/- 1.6 vs 32.0 +/- 2.8 pg digoxin equivalents/ml, with and without acute saline loading, respectively, p less than 0.01). These results indicate that the major substances detected by digoxin-like immunoreactivity and direct Na+,K+-ATPase inhibitory activity are completely different, at least in rat plasma.

摘要

我们通过两种不同的方法测量了急性输注生理盐水期间大鼠血浆中的内源性洋地黄样因子（EDF）。在生理盐水负荷期间，大鼠血浆中的Na +，K + -ATP酶抑制活性显著增加（分别为7.8±2.2% 和2.5±0.9%，有和没有急性生理盐水负荷时，p<0.05）。另一方面，急性生理盐水负荷期间血浆地高辛样免疫反应性显著降低（分别为16.9±1.6和32.0±2.8 pg地高辛当量/ml，有和没有急性生理盐水负荷时，p<0.01）。这些结果表明，至少在大鼠血浆中，通过地高辛样免疫反应性和直接Na +，K + -ATP酶抑制活性检测到的主要物质是完全不同的。