Goto A, Yamada K, Ishii M, Yoshioka M, Ishiguro T, Eguchi C, Sugimoto T
Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Biochem Biophys Res Commun. 1988 Apr 15;152(1):322-7. doi: 10.1016/s0006-291x(88)80717-4.
We attempted to purify a digitalis-like factor from volume expanded dog plasma using an inhibitory effect on the binding of [3H]ouabain to intact human erythrocytes to monitor digitalis-like activity. A highly polar [3H] ouabain displacing compound was purified to a high degree using a combination of chromatographic procedures including reverse phase and gel filtration high performance liquid chromatography. This compound, a reversible inhibitor of [3H]ouabain binding, closely resembles ouabain in its polarity and significantly increases during saline infusion. Its molecular weight was estimated to be 343Da. Moreover, similar compound was consistently detected in other mammalian plasma.
我们试图从容量扩张的犬血浆中纯化一种类洋地黄因子，利用其对[3H]哇巴因与完整人红细胞结合的抑制作用来监测类洋地黄活性。通过包括反相和凝胶过滤高效液相色谱在内的多种色谱方法联用，一种高极性的[3H]哇巴因置换化合物被高度纯化。这种化合物是[3H]哇巴因结合的可逆抑制剂，其极性与哇巴因非常相似，并且在输注生理盐水期间显著增加。其分子量估计为343Da。此外，在其他哺乳动物血浆中也一直能检测到类似的化合物。
