Department of Human Intelligence Systems, Graduate School of Life Science and Systems, Engineering, Kyushu Institute of Technology (Kyutech), 2-4 Hibikino, Wakamatsu, Kitakyushu, 808-0196, Japan.
Nanotechnology & Catalysis Research Centre (NANOCAT), IPS Building, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Sci Rep. 2017 May 9;7(1):1623. doi: 10.1038/s41598-017-01855-5.
A method to fabricate a bioinspired nanobiosensor using electronic-based artificial taste receptors for glucose diagnosis is presented. Fabricated bioinspired glucose nanobiosensor designated based on an artificial taste bud including an amperometric glucose biosensor and taste bud-inspired circuits. In fact, the design of the taste bud-inspired circuits was inspired by the signal-processing mechanism of taste nerves which involves two layers. The first, known as a type II cell, detects the glucose by glucose oxidase and transduces the current signal obtained for the pulse pattern is conducted to the second layer, called type III cell, to induce synchronisation of the neural spiking activity. The oscillation results of fabricated bioinspired glucose nanobiosensor confirmed an increase in the frequency of the output pulse as a function of the glucose concentration. At high glucose concentrations, the bioinspired glucose nanobiosensor showed a pulse train of alternating short and long interpulse intervals. A computational analysis performed to validate the hypothesis, which was successfully reproduced the alternating behaviour of bioinspired glucose our nanobiosensor by increasing the output frequency and alternation of pulse intervals according to the reduction in the resistivity of the biosensor.
提出了一种使用基于电子的人工味觉感受器制造仿生纳米生物传感器的方法，用于葡萄糖诊断。设计的仿生葡萄糖纳米生物传感器基于人工味蕾，包括安培葡萄糖生物传感器和受味觉启发的电路。实际上，受味觉启发的电路的设计灵感来自味觉神经的信号处理机制，该机制涉及两层。第一层，称为 II 型细胞，通过葡萄糖氧化酶检测葡萄糖，并将获得的电流信号转换为脉冲模式，传输到第二层，称为 III 型细胞，以诱导神经放电活动的同步。所设计的仿生葡萄糖纳米生物传感器的振荡结果证实，输出脉冲的频率随着葡萄糖浓度的增加而增加。在高葡萄糖浓度下，仿生葡萄糖纳米生物传感器显示出短和长脉冲间隔交替的脉冲串。进行了计算分析以验证假设，该假设成功地通过根据生物传感器电阻的降低增加输出频率和脉冲间隔的交替，再现了仿生葡萄糖纳米生物传感器的交替行为。
