Kharazmi A, Nielsen H, Bendtzen K
State Serum Institute, Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.
Immunobiology. 1988 Sep;177(4-5):363-70. doi: 10.1016/S0171-2985(88)80004-4.
The effects of recombinant TNF and GM-CSF on human peripheral blood neutrophil and monocyte chemotaxis and the superoxide response were studied. TNF exhibited a slight chemotactic activity for both cell types. Preincubation of neutrophils with as little as 40 units/ml strongly inhibited the neutrophil chemotaxis towards f-Met-Leu-Phe. The inhibition of monocyte chemotaxis required higher concentrations of TNF (greater than 400 units/ml). TNF at concentrations higher than 500 units/ml enhanced the generation of superoxide anions by neutrophils stimulated with f-Met-Leu-Phe. In contrast, TNF even at 2,000 units/ml did not prime monocytes for enhanced superoxide response. GM-CSF alone did not exhibit any chemotactic activity for any of the cell types tested. Preincubation of cells with GM-CSF inhibited chemotaxis of neutrophils but not of monocytes. GM-CSF was as potent as TNF in enhancing the generation of superoxide response by neutrophils. However, GM-CSF did not have any effect on monocyte superoxide response. The priming ability of TNF and GM-CSF on neutrophils was heat-sensitive. We conclude that TNF and GM-CSF play a more pronounced regulatory role on neutrophils than on monocytes. Inhibition of neutrophil chemotaxis followed by enhancement of the superoxide response by TNF and GM-CSF may provide an attractive mechanism by which these cytokines assist in fighting invading microorganisms.
研究了重组肿瘤坏死因子（TNF）和粒细胞-巨噬细胞集落刺激因子（GM-CSF）对人外周血中性粒细胞和单核细胞趋化性及超氧化物反应的影响。TNF对两种细胞类型均表现出轻微的趋化活性。用低至40单位/毫升的TNF预孵育中性粒细胞，可强烈抑制其对f-甲硫氨酰-亮氨酰-苯丙氨酸（f-Met-Leu-Phe）的趋化性。抑制单核细胞趋化性需要更高浓度的TNF（大于400单位/毫升）。浓度高于500单位/毫升的TNF可增强f-Met-Leu-Phe刺激的中性粒细胞超氧阴离子的生成。相反，即使浓度为2000单位/毫升的TNF也不能使单核细胞对超氧化物反应增强。单独的GM-CSF对所测试的任何细胞类型均未表现出任何趋化活性。用GM-CSF预孵育细胞可抑制中性粒细胞的趋化性，但不能抑制单核细胞的趋化性。GM-CSF在增强中性粒细胞超氧化物反应生成方面与TNF一样有效。然而，GM-CSF对单核细胞超氧化物反应没有任何影响。TNF和GM-CSF对中性粒细胞的启动能力对热敏感。我们得出结论，TNF和GM-CSF对中性粒细胞的调节作用比对单核细胞更为明显。TNF和GM-CSF先抑制中性粒细胞趋化性，随后增强其超氧化物反应，这可能提供了一种有吸引力的机制，通过该机制这些细胞因子协助对抗入侵的微生物。
