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黄芪多糖改善过氧化氢诱导的人脐静脉内皮细胞损伤。

Astragalus polysaccharide ameliorates H2O2-induced human umbilical vein endothelial cell injury.

作者信息

Han Ronghui, Tang Futian, Lu Meili, Xu Chonghua, Hu Jin, Mei Meng, Wang Hongxin

机构信息

Key Laboratory of Cardiovascular and Cerebrovascular Drug Research of Liaoning Province, Drug Research Institute, Jinzhou Medical University, Jinzhou, Liaoning 121001, P.R. China.

Department of Pharmacology, Key Laboratory of Cardiovascular and Cerebrovascular Drug Research of Liaoning Drug Research Institute, Jinzhou Medical University, Jinzhou, Liaoning 121001, P.R. China.

出版信息

Mol Med Rep. 2017 Jun;15(6):4027-4034. doi: 10.3892/mmr.2017.6515. Epub 2017 Apr 26.

DOI:10.3892/mmr.2017.6515
PMID:28487940
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5436204/
Abstract

Endothelial dysfunction caused by reactive oxygen species (ROS) has been implicated in numerous cardiovascular diseases. Astragalus polysaccharide (APS), an important bioactive component extracted from the Chinese herb Astragalus membranaceus, has been widely used for the treatment of cardiovascular disease. The present study aimed to investigate the effects of APS on hydrogen peroxide (H2O2)‑induced human umbilical vein endothelial cell (HUVEC) injury. Following treatment with 400 µM H2O2 for 24 h, cell viability was decreased and apoptosis was increased. However, pretreatment with APS for 1 h significantly attenuated H2O2‑induced injury in HUVECs. In addition, APS decreased intracellular ROS levels, increased the protein expression of endothelial nitric oxide synthase and copper‑zinc superoxide dismutase, elevated intracellular cyclic guanosine monophosphate (an activity marker for nitric oxide) levels and restored the mitochondrial membrane potential, compared with cells treated with H2O2 only. In conclusion, the results of the present study suggested that APS may protect HUVECs from injury induced by H2O2 via increasing the cell antioxidant capacity and nitric oxide (NO) bioavailability, which may contribute to the improvement of the imbalance between ROS and NO levels.

摘要

活性氧（ROS）引起的内皮功能障碍与多种心血管疾病有关。黄芪多糖（APS）是从中药黄芪中提取的一种重要生物活性成分，已被广泛用于治疗心血管疾病。本研究旨在探讨APS对过氧化氢（H2O2）诱导的人脐静脉内皮细胞（HUVEC）损伤的影响。用400µM H2O2处理24小时后，细胞活力降低，凋亡增加。然而，用APS预处理1小时可显著减轻H2O2诱导的HUVEC损伤。此外，与仅用H2O2处理的细胞相比，APS降低了细胞内ROS水平，增加了内皮型一氧化氮合酶和铜锌超氧化物歧化酶的蛋白表达，提高了细胞内环磷酸鸟苷（一氧化氮的活性标志物）水平，并恢复了线粒体膜电位。总之，本研究结果表明，APS可能通过提高细胞抗氧化能力和一氧化氮（NO）生物利用度来保护HUVEC免受H2O2诱导的损伤，这可能有助于改善ROS与NO水平之间的失衡。

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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fee1/5436204/8f89b7c54d54/MMR-15-06-4027-g02.jpg
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