Lee M S, Chang K S, Trujillo J M, McCredie K B, Keating M J, Freireich E J, Stass S A
Division of Laboratory Medicine, University of Texas System Cancer Center, Houston 77030.
Hematol Pathol. 1987;1(2):93-8.
The T-cell receptor gamma chain (TcR gamma) gene is rearranged in early T-cell differentiation. However, rearrangement of the TcR gamma gene is not specific for T lineage since it has also been noted in B-cell neoplasia. TcR beta gene rearrangement and heavy chain immunoglobulin gene rearrangement have also been reported in acute myelogenous leukemia (AML). Because of the previous reports of lineage heterogeneity at the molecular level, we analyzed seven patients who met the standard criteria for AML by Southern blot hybridization with a TcR gamma gene probe, a TcR beta gene probe, and an immunoglobulin heavy chain JH gene probe. In two samples, the TcR gamma gene was rearranged. One of these two samples also had rearrangement of the immunoglobulin JH gene. None of the seven samples showed TcR beta gene rearrangement. The two samples with TcR gamma gene rearrangement also showed 25% and 80% of terminal deoxynucleotidyl transferase (TdT) positivity, respectively. Both samples expressed myeloid lineage-associated antigens without any lymphoid lineage-associated antigens. Our studies indicate that TcR gamma gene rearrangement is not specific for lymphoid lineage and the presence of TcR gamma and immunoglobulin JH gene rearrangements without expression of lymphoid lineage-associated markers supports the concept that there is lymphoid lineage prematurity in AML.
T细胞受体γ链（TcRγ）基因在T细胞早期分化过程中发生重排。然而，TcRγ基因重排并非T细胞系所特有，因为在B细胞肿瘤中也有发现。急性髓性白血病（AML）中也报道了TcRβ基因重排和重链免疫球蛋白基因重排。鉴于此前在分子水平上关于谱系异质性的报道，我们用TcRγ基因探针、TcRβ基因探针和免疫球蛋白重链JH基因探针通过Southern印迹杂交分析了7例符合AML标准的患者。在两个样本中，TcRγ基因发生了重排。这两个样本中的一个还存在免疫球蛋白JH基因重排。7个样本均未显示TcRβ基因重排。两个发生TcRγ基因重排的样本中，末端脱氧核苷酸转移酶（TdT）阳性率分别为25%和80%。两个样本均表达髓系相关抗原，而无任何淋巴系相关抗原。我们的研究表明，TcRγ基因重排并非淋巴系所特有，且存在TcRγ和免疫球蛋白JH基因重排但无淋巴系相关标志物表达，这支持了AML中存在淋巴系早熟的概念。
