Hichri R, Amor H, Khammari M, Harzallah M, El Fekih S, Saad A, Ajina M, Ben Ali H
Department of Cytogenetics, Genetics and Molecular Biology of Human Reproduction, CHU Farhat Hached, Sousse, Tunisia.
Reproductive Medicine Unit, CHU Farhat Hached, Sousse, Tunisia.
Andrologia. 2018 Feb;50(1). doi: 10.1111/and.12813. Epub 2017 May 10.
The principal aim of this retrospective study was to examine the relationship between sperm apoptotic biomarkers and the patient's biclinical characteristics, the conventional sperm parameters and the results of assisted reproductive technology. Sperm analysis, activated caspases, annexin V staining for phosphatidylserine (PS) externalisation and labelling assay for DNA fragmentation were assessed in 122 males of infertile couples. Fifty-seven couples were allocated to the natural conception group, and 65 couples underwent IVF or ICSI. Semen of IVF/ICSI patients showed a higher proportion of apoptotic spermatozoa in their spermatozoa when compared with a natural conception group (p < .05). Sperm apoptotic biomarkers correlated with age, FSH, and conventional sperm parameters. DNA fragmentation correlated positively with the percentage of semen having externalised PS (r = .78, p = 0) and activated caspases (r = .71, p = 0). Patients without clinical pregnancy had higher frequency of DNA fragmentation, externalised PS and activated caspases compared to patients with clinical pregnancy (p < .001). The best specificity and greater sensitivity were obtained with the test of the DNA fragmentation compared to the other biomarkers. Among the apoptotic biomarkers, only DNA fragmentation was found to predict natural or assisted pregnancy better than conventional sperm parameters.
这项回顾性研究的主要目的是探讨精子凋亡生物标志物与患者的临床特征、传统精子参数以及辅助生殖技术结果之间的关系。对122对不育夫妇中的男性进行了精子分析、活化半胱天冬酶检测、磷脂酰丝氨酸（PS）外化的膜联蛋白V染色以及DNA片段化标记检测。57对夫妇被分配到自然受孕组，65对夫妇接受了体外受精（IVF）或卵胞浆内单精子注射（ICSI）。与自然受孕组相比，IVF/ICSI患者精液中的凋亡精子比例更高（p<0.05）。精子凋亡生物标志物与年龄、卵泡刺激素（FSH）和传统精子参数相关。DNA片段化与PS外化精液的百分比呈正相关（r=0.78，p=0），与活化半胱天冬酶呈正相关（r=0.71，p=0）。与临床妊娠患者相比，未临床妊娠患者的DNA片段化、PS外化和活化半胱天冬酶频率更高（p<0.001）。与其他生物标志物相比，DNA片段化检测具有最佳的特异性和更高的敏感性。在凋亡生物标志物中，只有DNA片段化在预测自然或辅助妊娠方面比传统精子参数表现更好。
