Urology Clinic, University of Pécs Clinical Centre, 7621 Pécs, Hungary.
National Laboratory on Human Reproduction, University of Pécs, 7624 Pécs, Hungary.
Int J Mol Sci. 2024 Mar 24;25(7):3627. doi: 10.3390/ijms25073627.
Magnetic activated cell sorting (MACS) is a well-known sperm selection technique, which is able to remove apoptotic spermatozoa from semen samples using the classic annexinV based method. Leukocytes and erythrocytes in semen samples or in testicular tissue processed for in vitro fertilization (IVF) could exert detrimental effects on sperm. In the current study, we rethought the aforementioned technique and used magnetic microbeads conjugated with anti-CD45/CD235a antibodies to eliminate contaminating leukocytes and erythrocytes from leukocytospermic semen samples and testicular tissue samples gained via testicular sperm extraction (TESE). With this technique, a 15.7- and a 30.8-fold reduction could be achieved in the ratio of leukocytes in semen and in the number of erythrocytes in TESE samples, respectively. Our results show that MACS is a method worth to reconsider, with more potential alternative applications. Investigations to find molecules labeling high-quality sperm population and the development of positive selection procedures based on these might be a direction of future research.
磁性激活细胞分选 (MACS) 是一种众所周知的精子选择技术，它能够使用经典的膜联蛋白 V 为基础的方法从精液样本中去除凋亡的精子。精液样本或用于体外受精 (IVF) 的睾丸组织中的白细胞和红细胞可能对精子产生有害影响。在本研究中，我们重新思考了上述技术，并使用与抗 CD45/CD235a 抗体偶联的磁性微珠，从白细胞精液样本和通过睾丸精子提取 (TESE) 获得的睾丸组织样本中去除污染的白细胞和红细胞。通过这种技术，可以将精液中白细胞的比例和 TESE 样本中红细胞的数量分别降低 15.7 倍和 30.8 倍。我们的结果表明，MACS 是一种值得重新考虑的方法，具有更多潜在的替代应用。寻找标记高质量精子群体的分子并基于这些分子开发正向选择程序可能是未来研究的一个方向。
