Landry M L, Zibello T A
Virology Reference Laboratory, Veterans Administration Medical Center, West Haven, Connecticut 06516.
J Infect Dis. 1988 Dec;158(6):1220-6. doi: 10.1093/infdis/158.6.1220.
Guinea pigs were infected intravaginally with either herpes simplex virus type 1 (HSV-1) or type 2 (HSV-2). One month postinoculation, animals were inoculated with the heterologous HSV type and observed for clinical disease and shedding of virus. One month after superinfection, animals were killed, and tissues were cocultivated to detect latent virus. Although the severity of clinical disease and the degree of shedding of virus were greatly reduced by prior infection with the heterologous virus type, superinfection did occur in 82%-90% of animals. Nervous system latency with the superinfecting virus was established in 20% of animals superinfected with HSV-2 and 55% superinfected with HSV-1. Of 21 animals tested, 5 had latent infection with both viruses, 6 with the superinfecting virus only, and 6 with the initial virus only. Protection from nervous system latency with the superinfecting virus correlated best with levels of serum neutralizing HSV antibody before superinfection.
豚鼠经阴道接种1型单纯疱疹病毒（HSV-1）或2型单纯疱疹病毒（HSV-2）。接种后1个月，给动物接种异源HSV型病毒，并观察其临床疾病和病毒脱落情况。再次感染1个月后，处死动物，将组织进行共培养以检测潜伏病毒。尽管先前感染异源病毒型可使临床疾病的严重程度和病毒脱落程度大大降低，但82%-90%的动物仍发生了再次感染。在感染HSV-2的动物中，20%建立了感染病毒的神经系统潜伏，在感染HSV-1的动物中，55%建立了感染病毒的神经系统潜伏。在接受检测的21只动物中，5只同时感染了两种病毒，6只仅感染了再次感染的病毒，6只仅感染了初始病毒。预防感染病毒的神经系统潜伏与再次感染前血清中和HSV抗体水平的相关性最佳。
