Department of Medical Ultrasound, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Guangdong Key Laboratory of Liver Disease Research, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Am J Transplant. 2017 Dec;17(12):3087-3097. doi: 10.1111/ajt.14345. Epub 2017 Jun 8.
The aim of the present study was to quantitatively diagnose and monitor the therapy response of hepatic ischemia-reperfusion injury (IRI) with the use of targeted ultrasound (US) imaging. Targeted microbubbles (MBs) were fabricated, and the binding of intracellular adhesion molecule 1 (ICAM-1) antibodies to MBs was observed. To establish a quantitative method based on targeted US imaging, contrast-enhanced US was applied for IRI rats. After andrographolide treatment, the IRI rats were subjected to the quantitative targeted US imaging for a therapeutic effect. Effective binding of ICAM-1 antibodies to MBs was observed. According to the quantitative targeted US imaging, the ICAM-1 normalized intensity difference (NID) in the IRI rats (38.74 ± 15.08%) was significantly higher than that in the control rats (10.08 ± 2.52%, p = 0.048). Further, different degrees of IRI (mild IRI, moderate to severe IRI) were distinguished by the use of the NID (37.14 ± 2.14%, 22.34 ± 1.08%, p = 0.002). Analysis of mRNA expression demonstrated the accuracy of analyzing the NID by using quantitative targeted US imaging (R = 0.7434, p < 0.001). Andrographolide treatment resulted in an obviously weakened NID of ICAM-1 (17.7 ± 4.8% vs 34.2 ± 6.6%, p < 0.001). The study showed the potential of the quantitative targeted US imaging method for the diagnosis and therapeutic monitoring of IRI.
本研究旨在通过使用靶向超声（US）成像技术对肝缺血再灌注损伤（IRI）进行定量诊断和监测治疗反应。我们制备了靶向微泡（MB），并观察了细胞间黏附分子 1（ICAM-1）抗体与 MB 的结合情况。为建立基于靶向 US 成像的定量方法，我们对 IRI 大鼠进行了对比增强 US 检查。在穿心莲内酯治疗后，对 IRI 大鼠进行了定量靶向 US 成像以评估治疗效果。观察到 ICAM-1 抗体与 MB 的有效结合。根据定量靶向 US 成像，IRI 大鼠的 ICAM-1 归一化强度差异（NID）（38.74 ± 15.08%）明显高于对照组大鼠（10.08 ± 2.52%，p = 0.048）。此外，还通过 NID 区分了不同程度的 IRI（轻度 IRI、中重度 IRI）（37.14 ± 2.14%、22.34 ± 1.08%，p = 0.002）。mRNA 表达分析表明，定量靶向 US 成像分析 NID 的准确性较高（R = 0.7434，p < 0.001）。穿心莲内酯治疗后，ICAM-1 的 NID 明显减弱（17.7 ± 4.8% 比 34.2 ± 6.6%，p < 0.001）。该研究表明，定量靶向 US 成像方法在 IRI 的诊断和治疗监测方面具有潜力。
