Department of Ultrasound, Tianjin First Center Hospital, Tianjin 300192, China.
Department of Ultrasound, Tianjin First Center Hospital, Tianjin 300192, China.
Ultrasound Med Biol. 2020 Sep;46(9):2464-2471. doi: 10.1016/j.ultrasmedbio.2020.04.021. Epub 2020 Jun 15.
This study aimed to investigate whether viscoelasticity measurements can be used to quantitatively analyze and monitor therapy response in hepatic ischemia-reperfusion injury (HIRI). All animals were divided into three groups: a sham operation group (n = 12), an ischemia-reperfusion injury (IRI) group (n = 12) and an andrographolide pre-treatment group (n = 6). To assess the feasibility of using shear-wave velocity (SWV) and shear-wave dispersion (SWD), shear-wave ultrasound elastography was applied onto IRI rats after 4 and 24 h of reperfusion or sham operation (each time point subgroup n = 6). For the verification experiments, six additional rats received andrographolide injection 2 h before IRI and were examined 24 h after reperfusion. The rats were sacrificed for biochemical and histopathological analyses after ultrasound scanning was performed. Compared with the sham group, the IRI group exhibited significantly higher SWD after both 4 and 24 h of reperfusion（10.69 ± 0.69 vs. 15.20 ± 3.23 and 9.01 ± 0.46 vs. 19.35 ± 0.86; p < 0.05). A positive correlation was found between SWD values and Suzuki's score (r = 0.621; p < 0.05). No correlation was found between SWV and Suzuki's score (r = 0.283; p > 0.05), although significant differences were found between the two groups after 24 h of reperfusion. Andrographolide treatment resulted in a significantly decreased SWD (15.24 ± 0.45 vs. 19.35 ± 0.86; p < 0.05), whereas SWV showed no statistically significant difference. This study demonstrated the potential of using viscoelasticity measurements for the diagnosis and therapeutic monitoring of HIRI, and that the use of SWD was significantly more advantageous than SWV.
本研究旨在探讨黏弹性测量是否可用于定量分析和监测肝缺血再灌注损伤（HIRI）的治疗反应。所有动物分为三组：假手术组（n = 12）、缺血再灌注损伤（IRI）组（n = 12）和穿心莲内酯预处理组（n = 6）。为评估剪切波速度（SWV）和剪切波分散度（SWD）的可行性，在再灌注后 4 和 24 小时对 IRI 大鼠应用剪切波超声弹性成像，每个时间点亚组 n = 6。为了验证实验，另外 6 只大鼠在 IRI 前 2 小时接受穿心莲内酯注射，并在再灌注后 24 小时进行检查。在进行超声扫描后，处死大鼠进行生化和组织病理学分析。与假手术组相比，IRI 组在再灌注后 4 和 24 小时的 SWD 显著升高（10.69 ± 0.69 vs. 15.20 ± 3.23 和 9.01 ± 0.46 vs. 19.35 ± 0.86；p < 0.05）。SWD 值与 Suzuki 评分呈正相关（r = 0.621；p < 0.05）。SWV 与 Suzuki 评分之间无相关性（r = 0.283；p > 0.05），尽管在再灌注 24 小时后两组之间存在显著差异。穿心莲内酯治疗导致 SWD 显著降低（15.24 ± 0.45 vs. 19.35 ± 0.86；p < 0.05），而 SWV 无统计学差异。本研究表明，黏弹性测量具有用于诊断和治疗监测 HIRI 的潜力，并且使用 SWD 明显优于 SWV。
