Research Center for Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan.
Department of Materials Science and Engineering, Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8577, Japan.
Adv Healthc Mater. 2017 Jul;6(14). doi: 10.1002/adhm.201700317. Epub 2017 May 10.
Nanostructured materials have drawn a broad attention for their applications in biomedical fields. Ligand-modified nanomaterials can well mimic the dynamic extracellular matrix (ECM) microenvironments to regulate cell functions and fates. Herein, ECM mimetic gold nanoparticles (Au NPs) with tunable surface arginine-glycine-aspartate (RGD) density are designed and synthesized to induce the chondrogenic differentiation of human mesenchymal stem cells (hMSCs). The biomimetic Au NPs with an average size of 40 nm shows good biocompatibility without affecting the cell proliferation in the studied concentration range. The RGD motifs on Au NPs surface facilitate cellular uptake of NPs into monolayer hMSCs through integrin-mediated endocytosis. The biomimetic NPs have a promotive effect on cartilaginous matrix production and marker gene expression in cell pellet culture, especially for the biomimetic Au NPs with high surface RGD density. This study provides a novel strategy for fabricating biomimetic NPs to regulate cell differentiation, which holds great potentials in tissue engineering and biomedical applications.
纳米结构材料因其在生物医学领域的应用而引起了广泛关注。配体修饰的纳米材料可以很好地模拟动态细胞外基质（ECM）微环境，从而调节细胞功能和命运。本文设计并合成了表面精氨酸-甘氨酸-天冬氨酸（RGD）密度可调的 ECM 模拟金纳米颗粒（Au NPs），以诱导人间充质干细胞（hMSCs）的软骨分化。平均尺寸为 40nm 的仿生 Au NPs 具有良好的生物相容性，在研究的浓度范围内不会影响细胞增殖。Au NPs 表面上的 RGD 基序通过整联蛋白介导的内吞作用促进 NPs 进入单层 hMSCs 的细胞摄取。仿生 NPs 在细胞球培养中对软骨基质的产生和标记基因表达具有促进作用，特别是对于表面 RGD 密度高的仿生 Au NPs。本研究为制备仿生 NPs 以调节细胞分化提供了一种新策略，在组织工程和生物医学应用中具有很大的潜力。
